Springer protocols feed by Cell Biology 
This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.
This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Introductory Review of Computational Cell Cycle Modeling
Recent advances in the modeling of the cell cycle through computer simulation demonstrate the power of systems biology. By definition, systems biology has the goal to connect a parts list, prioritized through experimental observation or high-throughput screens, by the topology of interactions defining intracellular networks to predict system function. Computer modeling of biological systems is often compared to a process of reverse engineering. Indeed, designed or engineered technical systems share many systems-level properties with biological systems; thus studying biological systems within an engineering framework has pr...
Source: Springer protocols feed by Cell Biology - June 10, 2014 Category: Cytology Source Type: news
The Roles of Cohesins in Mitosis, Meiosis, and Human Health and Disease
Mitosis and meiosis are essential processes that occur during development. Throughout these processes, cohesion is required to keep the sister chromatids together until their separation at anaphase. Cohesion is created by multiprotein subunit complexes called cohesins. Although the subunits differ slightly in mitosis and meiosis, the canonical cohesin complex is composed of four subunits that are quite diverse. The cohesin complexes are also important for DNA repair, gene expression, development, and genome integrity. Here we provide an overview of the roles of cohesins during these different events as well as their roles ...
Source: Springer protocols feed by Cell Biology - June 10, 2014 Category: Cytology Source Type: news
Cell Cycle Regulation During Viral Infection
To replicate their genomes in cells and generate new progeny, viruses typically require factors provided by the cells that they have infected. Subversion of the cellular machinery that controls replication of the infected host cell is a common activity of many viruses. Viruses employ different strategies to deregulate cell cycle checkpoint controls and modulate cell proliferation pathways. A number of DNA and RNA viruses encode proteins that target critical cell cycle regulators to achieve cellular conditions that are beneficial for viral replication. Many DNA viruses induce quiescent cells to enter the cell cycle; this is...
Source: Springer protocols feed by Cell Biology - June 10, 2014 Category: Cytology Source Type: news
Embryo Transfer
Embryo transfer is a critical step in the overall success of in vitro fertilization (IVF). Despite its apparent simplicity, it is an integral part of the IVF cycle that can be difficult to teach and perform well. In this chapter we describe the procedure of embryo transfer and discuss various modifications that have been evaluated in an effort to improve the probability of embryo implantation including ultrasound guidance, catheter design, transfer technique, as well as enrichment of the embryo transfer media. (Source: Springer protocols feed by Cell Biology)
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
Embryo Selection Using Metabolomics
Faced with an increasing demand to select one embryo to transfer back to patients, a number of techniques are being developed to assist in discriminating differences within the cohort of a patient’s embryos. A new and emerging technology which allows us to measure the profile of different metabolites in embryo culture media and formulate a viability score correlated to implantation potential is metabolomics. (Source: Springer protocols feed by Cell Biology)
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
Embryo Culture and Selection: Morphological Criteria
In this chapter, we have outlined the various morphological criteria for selection of the best embryo at each important milestone encountered in the progress from the oocyte to the blastocyst. As Gerris et al. stated, a combination of one, two, or even three selection points should lead to a more accurate selection of the best embryo, as no one criterion is better than the other. An embryo that fails to meet the entire set of selection criteria must be avoided as culture cannot correct an impaired embryo. (Source: Springer protocols feed by Cell Biology)
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
Detection of Monogenic Disorders and Chromosome Aberrations by Preimplantation Genetic Diagnosis
This chapter highlights the methodologies of single cell genetic diagnosis along with the strengths and weaknesses of existing techniques. (Source: Springer protocols feed by Cell Biology)
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
Ovarian Tissue Cryopreservation and Transplantation: A Realistic, Effective Technology for Fertility Preservation
It is clear that ovarian tissue cryopreservation can serve a very important role in providing fertility preservation. To date, more than 30 live human births have resulted from the transplantation of cryopreserved ovarian tissue, proving the successful implementation of this technique. The ideal conditions for ovarian tissue cryopreservation have yet to be determined, indicating the crucial need for more research in this field. Nonetheless, it is recommended that ovarian tissue cryopreservation be offered as an option to women before undergoing chemotherapy treatment in instances where there is no time to delay for hormona...
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
Cryopreservation of Eggs
Oocyte cryopreservation is playing an increasingly important role in the field of human infertility treatment. The ability to store viable oocytes for later use has given many women the option to delay childbearing in order to pursue other ventures in life, without the concern of losing the opportunity to have a family. Furthermore, oocyte cryopreservation is very valuable for diseased patients who have to undergo treatments that may compromise fertility. Also, infertility patients who produce large numbers of oocytes during a retrieval cycle now have the option of storing some eggs prior to fertilization, thereby reducing...
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
Techniques for Slow Cryopreservation of Embryos
The slow cryopreservation of embryos has been used for nearly three decades as a means of storing surplus conceptuses from single IVF (in vitro fertilization) cycles. Doing so has allowed caregivers to maximize pregnancy rates without wastage of precious biological materials. Very detailed methods are described here using a popular biological freezing unit manufactured by Planer PLC (Middlesex, UK). Culture media preparation and tranfer protocols, including replacement in both natural and stimulated cycles, are included. (Source: Springer protocols feed by Cell Biology)
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
Treatment of Male Infertility
Major difficulties exist in the accurate and meaningful diagnosis of male reproductive dysfunction, and our understanding of the epidemiology and etiology of male infertility has proven quite complex. (Source: Springer protocols feed by Cell Biology)
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
Sperm Retrieval and Quality Evaluation
Technical refinements in sperm retrieval methods and the application of advanced reproductive technologies (ART) using surgically retrieved sperm have enabled biological paternity in azoospermic men who were considered untreatable 20 years ago. Achievement of optimal reproductive outcomes in these patients benefits greatly from a multistep, interdisciplinary process of sperm acquisition that involves reproductive endocrinologists, urologists, or other specialists in male subfertility, and laboratory personnel with expertise in characterizing and isolating sperm from surgically retrieved specimens. The critical steps in thi...
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
Oocyte Retrieval and Quality Evaluation
After more than 30 years of harvesting oocytes for in vitro fertilization, collection techniques are well established. Presented here are dependable methods for maintaining environmental stability during such procedures and general descriptions of the materials collected. (Source: Springer protocols feed by Cell Biology)
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
IVF Stimulation: Protocols for Poor Responders
One of the most vexing challenges in the practice of reproductive medicine is the management of the “poor responder,” specifically the patient manifesting an inadequate follicular response to ovarian stimulation. Poor response predicts a reduction in the number of mature oocytes retrieved, with the consequences of fewer embryos available for selection and transfer, reduced pregnancy rates, and a markedly decreased likelihood of residual embryos for cryopreservation. This chapter reviews the definition and prediction of poor response and discusses strategies that have been developed and incorporated into the rep...
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
Ovarian Stimulation for IVF: Mild Approaches
In contrast to current approaches, the aim of mild stimulation is to develop safer and more patient-friendly protocols in which the risks of the treatment as a whole are minimized. Mild stimulation is defined as the method when exogenous gonadotropins are administered at lower doses, and/or for a shorter duration in GnRH antagonist co-treated cycles, or when oral compounds (antiestrogens, aromatase inhibitors) are used for ovarian stimulation for IVF, with the aim of limiting the number of oocytes obtained to fewer than eight. In this chapter we discuss the relevant physiology of follicle development, the development of mi...
Source: Springer protocols feed by Cell Biology - May 1, 2014 Category: Cytology Source Type: news
