The art of proteomics translation
Publication date: 2013 Source:Translational Proteomics, Volume 1, Issue 1 Author(s): Jean-Charles Sanchez (Source: Translational Proteomics)
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Molecular mechanisms of increased cerebral vulnerability after repeated mild blast-induced traumatic brain injury
Publication date: Available online 2 December 2013 Source:Translational Proteomics Author(s): Alaa Kamnaksh , Farid Ahmed , Erzsebet Kovesdi , Erin S. Barry , Neil E. Grunberg , Joseph B. Long , Denes V. Agoston The consequences of a mild traumatic brain injury can be especially severe if it is repeated within the period of increased cerebral vulnerability (ICV) that follows the initial insult. To better understand the molecular mechanisms that contribute to ICV, we exposed rats to different levels of mild blast overpressure (5 exposures; total pressure range: 15.54–19.41psi or 107.14–133.83kPa) at a rate of...
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Reverse phase protein array based tumor profiling identifies a biomarker signature for risk classification of hormone receptor-positive breast cancer
In conclusion, the biomarker signature introduced supports RPPA as a tool for cancer biomarker discovery. Graphical abstract (Source: Translational Proteomics)
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Ultrahigh resolution profiles lead to more detailed serum peptidome signatures of pancreatic cancer
Publication date: March 2014 Source:Translational Proteomics, Volume 2 Author(s): Simone Nicolardi , Berit Velstra , Bart J. Mertens , Bert Bonsing , Wilma E. Mesker , Rob A.E.M. Tollenaar , André M. Deelder , Yuri E.M. van der Burgt Mass spectrometry-based (clinical) proteomics has been widely applied as a technology to find and validate disease-specific protein signatures. MALDI-based peptidome profiles provide a suitable platform for classification of body fluids or tissues, albeit at the cost of being unable to observe low abundant species. Here we show that a fully automated one-step solid-phase extractio...
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Profiling of human myotubes reveals an intrinsic proteomic signature associated with type 2 diabetes
Publication date: March 2014 Source:Translational Proteomics, Volume 2 Author(s): Lubna Al-Khalili , Thais de Castro Barbosa , Jörgen Östling , Julie Massart , Mutsumi Katayama , Ann-Christin Nyström , Jan Oscarsson , Juleen R. Zierath The development of insulin resistance and type 2 diabetes (T2D) involves a complex array of metabolic defects in skeletal muscle. An in vitro cell culture system excludes the acute effects of external systemic factors existing in vivo. Thus, we aimed to determine whether intrinsic differences in the protein profile exist in cultured myotubes derived from T2D versus normal gluc...
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Analysis of plasma from prostate cancer patients links decreased carnosine dipeptidase 1 levels to lymph node metastasis
Publication date: March 2014 Source:Translational Proteomics, Volume 2 Author(s): Ulrika Qundos , Henrik Johannesson , Claudia Fredolini , Gillian O’Hurley , Rui Branca , Mathias Uhlén , Fredrik Wiklund , Anders Bjartell , Peter Nilsson , Jochen M. Schwenk There is a need for a better differentiation of aggressive tumors in prostate cancer to design a tailored treatment for each patient, preferably by a minimally invasive analysis of blood samples. In a previous study, we discovered a decrease of plasma levels of carnosine dipeptidase 1 (CNDP1) in association with aggressive prostate cancer. Now this relat...
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Quality requirements for quantitative clinical chemistry proteomics
Publication date: March 2014 Source:Translational Proteomics, Volume 2 (Source: Translational Proteomics)
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Recent updates on drug abuse analyzed by neuroproteomics studies: Cocaine, Methamphetamine and MDMA
Publication date: Available online 2 May 2014 Source:Translational Proteomics Author(s): Firas Kobeissy , Tarek H. Mouhieddine , Amaly Nokkari , Muhieddine Itani , Mohammed Mouhieddine , Zhiqun Zhang , Rui Zhu , Mark S. Gold , Kevin K. Wang , Yehia Mechref Currently, drug abuse and addiction represent a global public health concern with about 13.6 million people using illicit drugs in the USA alone. Substance abuse intervenes in normal brain functioning, causing alterations in memory, behavior and neuronal physiology. Although many studies have been conducted to elucidate the mode of action of different drugs...
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Microwave and magnetic (M2) proteomics of a mouse model of mild traumatic brain injury
Publication date: June 2014 Source:Translational Proteomics, Volume 3 Author(s): Teresa M. Evans , Holly Van Remmen , Anjali Purkar , Swetha Mahesula , J.A.L. Gelfond , Marian Sabia , Wenbo Qi , Ai-Ling Lin , Carlos A. Jaramillo , William E. Haskins Short-term increases in oxidative stress and decreases in motor function, including debilitating effects on balance and motor control, can occur following primary mild traumatic brain injuries (mTBI). However, the long-term effects on motor unit impairment and integrity as well as the molecular mechanisms underlying secondary injuries are poorly understood. We hyp...
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Chemokines after human ischemic stroke: From neurovascular unit to blood using protein arrays
Publication date: June 2014 Source:Translational Proteomics, Volume 3 Author(s): Teresa García-Berrocoso , Dolors Giralt , Víctor Llombart , Alejandro Bustamante , Anna Penalba , Alan Flores , Marc Ribó , Carlos A. Molina , Anna Rosell , Joan Montaner Chemokines act mainly in guiding leukocyte migration along the endothelium. Apart from angiogenesis or neuronal survival, chemokines are involved in damage and repair of brain tissue after ischemic stroke. We studied the presence of chemokines directly in neurons and brain blood vessels that were obtained by means of laser microdissection from human ischemic ...
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Platelet proteomics in cardiovascular diseases
Publication date: Available online 7 October 2014 Source:Translational Proteomics Author(s): Paula Vélez , Ángel García In recent years, platelet proteomics has been applied successfully to the study of cardiovascular diseases (CVDs). It is very well known that platelets play a pivotal role in the pathophysiological mechanisms underlying many CVDs, especially acute coronary syndromes (ACSs), since they are implied in thrombus formation after atheroma plaque rupture. This is the reason why molecules involved in platelet activation and aggregation are primary targets for treatment of ACSs. Many efforts are aimed at ...
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Contribution of proteomics to the management of vascular disorders
Publication date: Available online 28 October 2014 Source:Translational Proteomics Author(s): Fernando de la Cuesta , Laura Mourino-Alvarez , Montserrat Baldan-Martin , Rafael Moreno-Luna , Maria G. Barderas Vascular disorders, and in particular atherothrombosis, are currently a leading cause of morbidity and mortality in Western societies. Proteomics research into these disorders has helped improving our knowledge of the underlying mechanisms involved in the development of atherothrombosis, as well as providing novel biomarkers to diagnose and for the prognosis of this disease. However, the application of these a...
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
The application of multiple reaction monitoring to assess ApoA-I methionine oxidations in diabetes and cardiovascular disease
In this study, we developed an MRM assay to monitor the abundance ratio of the peptide containing oxidized M148 to the native peptide in ApoA-I. Measurement of the oxidized-to-unoxidized-M148 ratio was reproducible (CV<5%). The extent of methionine M148 oxidation in the HDL of healthy controls, and type 2 diabetic participants with and without prior cardiovascular events (CVD) were then examined. The results suggest a significant increase in the relative ratio of the peptide containing oxidized M148 to the unmodified peptide in the HDL of participants with diabetes and CVD (p <0.001), compared to participants...
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
Proteomics as a new paradigm to tackle Parkinson’s disease research challenges
Publication date: December 2014 Source:Translational Proteomics, Volumes 4–5 Author(s): Virginie Licker , Pierre R. Burkhard Disease-modifying therapies capable to stop or slow Parkinson’s disease (PD) progression are still elusive due to severe shortcomings in the understanding of PD etiopathogenesis as well as limitations in routine clinically-based diagnosis precluding PD detection during its early course. Proteomics has recently emerged as one of the most attractive approaches to unravel the complex nature of PD processes and investigate PD potential biomarkers. In contrast to traditional candidate-based stud...
Source: Translational Proteomics - November 7, 2014 Category: Biomedical Science Source Type: research
