Transcription in the acetoin catabolic pathway is regulated by AcoR and CcpA in Bacillus thuringiensis
In this study, we analyzed transcription and regulation of the aco operon in Bacillus thuringiensis (Bt). RT-PCR analysis revealed that acoABCL forms one transcriptional unit. The Sigma 54 controlled consensus sequence was located 12 bp from the acoA transcriptional start site (TSS). β-galactosidase assay revealed that aco operon transcription is induced by acetoin, controlled by sigma 54, and positively regulated by AcoR. The HTH domain of AcoR recognized and specifically bound to a 13-bp inverted repeat region that participates in 30-bp fragment mapping 81 bp upstream of the acoA TSS. The GAF domain in AcoR represses en...
Source: Microbiological Research - February 13, 2020 Category: Infectious Diseases Source Type: research
Expression of attack and growth phase genes of Bdellovibrio bacteriovorus in the presence of Gram-negative and Gram-positive prey
Publication date: Available online 13 February 2020Source: Microbiological ResearchAuthor(s): M. Waso, S. Khan, W. Ahmed, W. KhanAbstractThe expression of attack phase (AP) and growth phase (GP) genes of Bdellovibrio bacteriovorus (B. bacteriovorus) was compared in the presence of Gram-negative [Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae)] and Gram-positive [Enterococcus faecium (E. faecium)] prey, using relative quantitative polymerase chain reaction (relative qPCR) assays. The genes bd0108 (pili retraction/extrusion) and merRNA (massively expressed riboswitch RNA) were highly expressed in the AP ...
Source: Microbiological Research - February 13, 2020 Category: Infectious Diseases Source Type: research
Modification of the host ubiquitome by bacterial enzymes
Publication date: Available online 11 February 2020Source: Microbiological ResearchAuthor(s): Jennifer Berglund, Rafaela Gjondrekaj, Ellen Verney, Julie Maupin-Furlow, Mariola J EdelmannAbstractAttachment of ubiquitin molecules to protein substrates is a reversible post-translational modification (PTM), which occurs ubiquitously in eukaryotic cells and controls most cellular processes. As a consequence, ubiquitination is an attractive target of pathogen-encoded virulence factors. Pathogenic bacteria have evolved multiple mechanisms to hijack the host’s ubiquitin system to their advantage. In this review, we discuss the b...
Source: Microbiological Research - February 12, 2020 Category: Infectious Diseases Source Type: research
Identification of a gene encoding the last step of the L-rhamnose catabolic pathway in Aspergillus niger revealed the inducer of the pathway regulator
In this study, we identified the gene (lkaA) involved in the conversion of L-2-keto-3-deoxyrhamnonate (L-KDR) into pyruvate and L-lactaldehyde, which is the last step of the Rha pathway. Deletion of lkaA resulted in impaired growth on L-rhamnose, and potentially in accumulation of L-KDR. Contrary to ΔlraA, ΔlrlA and ΔlrdA, the expression of the Rha-responsive genes that are under control of RhaR, were at the same levels in ΔlkaA and the reference strain, indicating the role of L-KDR as the inducer of the Rha pathway regulator. (Source: Microbiological Research)
Source: Microbiological Research - February 9, 2020 Category: Infectious Diseases Source Type: research
A novel strain of endophytic Streptomyces for the biocontrol of strawberry anthracnose caused by Glomerella cingulate
In this study, we aimed to isolate and screen endophytic actinobacteria, to identify potential biocontrol agents capable of suppressing strawberry anthracnose. A total of 226 actinobacteria were successfully isolated from surface-sterilized strawberry tissues. In the first screening, 217 out of 226 actinobacteria isolates were studied for their suppression effect on strawberry anthracnose using a detached leaflet assay. It was discovered that isolates MBFA-172 and MBFA-227 markedly suppressed the development of anthracnose lesions. The efficacy of both isolates was then tested on two-month-old strawberry plug seedlings in ...
Source: Microbiological Research - February 9, 2020 Category: Infectious Diseases Source Type: research
Unraveling the metabolite signature of citrus showing defense response towards Candidatus Liberibacter asiaticus after application of endophyte Bacillus subtilis L1-21
Publication date: Available online 31 January 2020Source: Microbiological ResearchAuthor(s): Shahzad Munir, Yongmei Li, Pengfei He, Pengbo He, Ayesha Ahmed, Yixin Wu, Yueqiu HeAbstractHuanglongbing (HLB) is one of the most serious citrus diseases, caused by phloem limited endophytic bacteria Candidatus Liberibacter asiaticus (Clas), affecting worldwide citrus production. Metabolomics approaches were employed to gain insight into mechanisms involved in defense against Clas in endophyte Bacillus subtilis L1-21 treated diseased and healthy citrus plants. Using LC-ESI-MS/MS, we compared the metabolic profile of citrus plants b...
Source: Microbiological Research - February 1, 2020 Category: Infectious Diseases Source Type: research
The rhizosphere signature on the cell motility, biofilm formation and secondary metabolite production of a plant-associated Lysobacter strain
Publication date: Available online 31 January 2020Source: Microbiological ResearchAuthor(s): Francesca Brescia, Martina Marchetti-Deschmann, Rita Musetti, Michele Perazzolli, Ilaria Pertot, Gerardo PuopoloAbstractLysobacter spp. are common bacterial inhabitants of the rhizosphere of diverse plant species. However, the impact of the rhizosphere conditions on their physiology is still relatively understudied. To provide clues on the behaviour of Lysobacter spp. in this ecological niche, we investigated the physiology of L. capsici AZ78 (AZ78), a biocontrol strain isolated from tobacco rhizosphere, on a common synthetic growt...
Source: Microbiological Research - January 31, 2020 Category: Infectious Diseases Source Type: research
Shotgun Proteomic Analysis of ESBL-Producing and Non-ESBL-Producing Klebsiella Pneumoniae Clinical Isolates
Publication date: Available online 25 January 2020Source: Microbiological ResearchAuthor(s): Shymaa Enany, Samira Zakeer, Ahmed A. SayedAbstractKlebsiella pneumoniae is a pathogenic bacterium that is responsible for a wide range of infections in humans. An increased rate of infections caused by multi-drug-resistant K. pneumoniae has been noted in the last two decades. The association between antimicrobial resistance and virulence is an important topic of study. Genomic tools have been used widely for the detection of virulence. In our study, we used proteomic analysis with mass spectrometry and bioinformatics tools to expl...
Source: Microbiological Research - January 26, 2020 Category: Infectious Diseases Source Type: research
Induction of drought tolerance in tomato upon the application of ACC deaminase producing plant growth promoting rhizobacterium Bacillus subtilis Rhizo SF 48
Publication date: Available online 25 January 2020Source: Microbiological ResearchAuthor(s): H.G. Gowtham, S. Brijesh Singh, M. Murali, N. Shilpa, Melvin Prasad, Mohammed Aiyaz, K.N. Amruthesh, S.R. NiranjanaAbstractA total of ten 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing PGPR isolates were selected and evaluated for the induction of drought stress tolerance in tomato. Among the selected PGPR, maximum seed (laboratory) and plant growth promotion (greenhouse) was observed in tomato seeds bacterized with Bacillus subtilis Rhizo SF 48. The genomic study confirmed the presence of ACC deaminase gene in Rhizo S...
Source: Microbiological Research - January 26, 2020 Category: Infectious Diseases Source Type: research
Metabolite profiling reveals a complex response of plants to application of plant growth-promoting endophytic bacteria
Publication date: Available online 25 January 2020Source: Microbiological ResearchAuthor(s): Ahmad Mahmood, Ryota KataokaAbstractEndophytic bacteria have been explored for their role in plant growth promotion, however, not much has been explored in cucumber. The metabolomic response of plants to application of such microbes also remains largely unknown. Thus, we investigated the application of endophytic bacteria to cucumber to infer their role in plant growth promotion and document metabolome response. The lowest healthy leaf-stalks were sampled from four differently sourced cucumber plants, and endophytic bacteria were i...
Source: Microbiological Research - January 26, 2020 Category: Infectious Diseases Source Type: research
The orphan histidine kinase PdtaS-p regulates both morphological differentiation and antibiotic biosynthesis together with the orphan response regulator PdtaR-p in Streptomyces
In this study, based on bioinformatics analysis, we identify the cognate RR PdtaR-p of PdtaS-p by phenotype similarity of gene deletion mutants as well as in vitro phosphor-transfer assay. We show that the mutants (ΔpdtaR-p and ΔpdtaS-p) exhibit almost the same phenotypical changes, showing a bald phenotype on MS agar and reduced pristinamycin biosynthesis. Further phosphor-transfer assay indicates that the phosphoryl group of HK PdtaS-p can be specifically transferred to RR PdtaR-p. Compared with the majority of RRs that harbor DNA-binding domains, PdtaR-p contains a putative ANTAR RNA-binding domain involved in control...
Source: Microbiological Research - January 16, 2020 Category: Infectious Diseases Source Type: research
Genotyping and population diversity of Bacillus anthracis in China based on MLVA and canSNP analysis
In this study, 191 isolates were selected to assess the diversity of B. anthracis in China. Five isolates were confirmed not to be B. anthracis by clustered regularly interspaced short palindromic repeat analysis, while the remaining 186 isolates were typed using canonical SNP (canSNP) and VNTR analyses. Five sublineages/subgroups, A.Br.001/002, A.Br.Vollum, A.Br.Aust.94, A.Br.Ames, and A.Br.008/009, were detected based on 13 canSNP sites. The 186 isolates were further assigned 114 sequence types based on 27 VNTR loci, with major branches correlating with the canSNP analysis. We then used a simplified multiple-locus variab...
Source: Microbiological Research - January 16, 2020 Category: Infectious Diseases Source Type: research
Pantoea Natural Product 3 is encoded by an eight-gene biosynthetic gene cluster and exhibits antimicrobial activity against multi-drug resistant Acinetobacter baumannii and Pseudomonas aeruginosa
Publication date: Available online 15 January 2020Source: Microbiological ResearchAuthor(s): Ashley N. Williams, John StavrinidesAbstractMulti-drug resistant Acinetobacter baumannii and Pseudomonas aeruginosa continue to pose a serious health threat worldwide. Two Pantoea agglomerans strains, 3581 and SN01080, produce an antibiotic effective against these pathogens. To identify the antibiotic biosynthetic gene clusters, independent genetic screens were conducted for each strain using a mini-Tn5 transposon, which resulted in the identification of the same conserved eight-gene cluster. We have named this antibiotic Pantoea N...
Source: Microbiological Research - January 16, 2020 Category: Infectious Diseases Source Type: research
Diversity of endophytic plant-growth microorganisms from Gentianella weberbaueri and Valeriana pycnantha, highland Peruvian medicinal plants
Publication date: Available online 15 January 2020Source: Microbiological ResearchAuthor(s): Rocío Ulloa-Muñoz, Percy Olivera-Gonzales, Alberto Castañeda-Barreto, Gretty K. Villena, Carmen Tamariz-AngelesAbstractMicrobial diversity in Peruvian mountain areas is poorly know, specially endophytic microorganisms of medicinal native plants from the Cordillera Blanca. So, nine bacterial and six fungal species were isolated from Gentianella weberbaueri and Valeriana pycnantha. According to 16S rDNA analysis, bacterial strains belong to genera Rahnella, Pseudomonas, Serratia, Rouxiella, and Bacillus; while ITS analysis showed ...
Source: Microbiological Research - January 16, 2020 Category: Infectious Diseases Source Type: research
Editorial Board
Publication date: February 2020Source: Microbiological Research, Volume 232Author(s): (Source: Microbiological Research)
Source: Microbiological Research - January 16, 2020 Category: Infectious Diseases Source Type: research
