Detection of virulence factors and antifungal susceptibility of human and avian Aspergillus flavus isolates
Publication date: Available online 12 September 2019Source: Journal de Mycologie MédicaleAuthor(s): Dhoha Ghorbel, Inès Hadrich, Sourour Neji, Houaida Trabelsi, Hatem Belaaj, Hayet Sellami, Fatma Cheikhrouhou, Fattouma Makni, Ali AyadiAbstractAspergillus flavus is the second leading cause of invasive and non-invasive aspergillosis. Secretion of hydrolytic enzymes is considered as a virulence factor in this species. Our work aimed to study in vitro production of some virulence factors, to evaluate the biofilm production against human and avian A. flavus isolates and to investigate the antifungal susceptibility agents. Hyd...
Source: Journal of Medical Mycology - September 13, 2019 Category: Biology Source Type: research
A computational study targeting the mutated L321F of ERG11 gene in C.albicans, associated with fluconazole resistance with bioactive compounds from Acacia nilotica
Conclusion: The findings of the study emphasize that kaempferol, apigenin and catechin from A.nilotica seem to possess a promising inhibitory effect against the wild and mutated ERG11 of C.albicans suggesting ERG11 as the best target to combat fluconazole resistant C.albicans with further in-vivo validation targeting the same. (Source: Journal of Medical Mycology)
Source: Journal of Medical Mycology - September 13, 2019 Category: Biology Source Type: research
Prototheca wickerhamii prepatellar bursitis in an immunocompetent woman: A case report
We report a patient who had no apparent immunodeficiency developed P. wickerhamii prepatellar bursitis following intra-bursal corticosteroid injection. Clinical manifestations could not distinguish Prototheca bursitis from septic bursitis caused by other pathogens. Bursal fluid aspiration sent for direct microscopic examination and cultures could give an early diagnosis. Systemic antifungal therapy with complete surgical excision of infected bursa provided a good outcome. (Source: Journal of Medical Mycology)
Source: Journal of Medical Mycology - September 13, 2019 Category: Biology Source Type: research
Human infections due to Schizophyllum commune: case report and review of the literature
Publication date: Available online 12 September 2019Source: Journal de Mycologie MédicaleAuthor(s): Caterina Cavanna, Fabio Pagella, Maria Carmela Esposto, Francesca Tamarozzi, Libera Clemente, Piero Marone, Elina Matti, Fabiola LallittoAbstractSchizophyllum commune is an environmental basidiomycetous fungus, causing occasional, predominantly respiratory, infections in humans. Although S. commune is considered an emerging pathogen, some authors pointed out the possibility that recent advances in diagnostic technologies now allow a more prompt and precise identification at the species level. Here we describe the first Ital...
Source: Journal of Medical Mycology - September 13, 2019 Category: Biology Source Type: research
Amyloidosis secondary to chronic pulmonary aspergillosis: case report and a systematic review of literature
Publication date: Available online 12 September 2019Source: Journal de Mycologie MédicaleAuthor(s): Dharmesh Patel, Ritesh Agarwal, Sahajal Dhooria, Umapati Hedge, Hardik Patel, Inderpaul Singh SehgalAbstractSecondary amyloidosis results from the deposition of abnormally folded proteins in body organs due to chronic inflammatory disorders. Kidneys are the most commonly affected organ and manifest as nephrotic syndrome with or without renal failure. Chronic pulmonary aspergillosis (CPA) is a chronic infection of lung parenchyma affecting those with an underlying structural lung disease. Herein, we present a case of CPA whe...
Source: Journal of Medical Mycology - September 13, 2019 Category: Biology Source Type: research
Genotyping of Candida albicans isolates from oropharyngeal candidiasis in head & neck cancer patients in Iran: Molecular epidemiology and SAP2 gene expression
Conclusion: We found few genetically-related as well as identical isolates among the 32 Candida strains which indicated low cross-contaminations among the patients. There was no relationship between C. albicans MLST profiles and their geographic distribution, cancer type and SAP2 gene expression. This lack of correlation was possibly due to the small understudy population; hence, finding more relevance requires studies with a higher number of samples. (Source: Journal of Medical Mycology)
Source: Journal of Medical Mycology - September 13, 2019 Category: Biology Source Type: research
Vulvovaginal Candidiasis among Symptomatic Women of Childbearing Age attended at a Medical Analysis Laboratory in Franceville, Gabon
Conclusion: This study offers the first estimation of VVC among Gabonese women in childbearing age with the symptoms. It showed that VVC is very common in Gabon. C. albicans as the most commonly represented species (Source: Journal of Medical Mycology)
Source: Journal of Medical Mycology - August 29, 2019 Category: Biology Source Type: research
Anti-fungal susceptibility and virulence factors of Candida spp. isolated from blood cultures
This study aims to investigate anti-fungal drug susceptibility, enzymatic activity and biofilm formation of the Candida spp. isolated from blood cultures. In 2016, a total of 84 clinical Candida isolates were analyzed for minimum inhibitory concentration (MIC) against fluconazole and amphotericin B by agar diffusion E-test (E-strips). Three enzymatic activity tests for phospholipase, proteinase and esterase were performed by using egg yolk agar, bovine serum albumin medium and Tween 80 opacity medium, respectively. Biofilm formation was determined by crystal violet staining. To describing the various Candida distributions ...
Source: Journal of Medical Mycology - August 23, 2019 Category: Biology Source Type: research
Saksenaea vasiformis infections: A case of an immunocompetent adult after mild injury and a literature review
Publication date: Available online 25 July 2019Source: Journal de Mycologie MédicaleAuthor(s): K. Samaras, A.-M. Markantonatou, D. Karapiperis, P. Digonis, N. Kartalis, N. Kostogloudis, T.-A. VyzantiadisAbstractSaksenaea vasiformis is an emerging human pathogen, belonging to the order Mucorales of the subphylum Mucormycotina, most often associated with rhino-cerebral, cutaneous and subcutaneous infections following trauma. A review of the published literature was attempted on the occasion of a cutaneous leg infection with favorable outcome in a young immunocompetent man after mild injury. The overall aim was the facilitat...
Source: Journal of Medical Mycology - August 22, 2019 Category: Biology Source Type: research
In vitro activity of carvacrol, cinnamaldehyde and thymol combined with antifungals against Malassezia pachydermatis
In conclusion, the results obtained indicate that the phytochemicals tested showed relevant in vitro anti-M. pachydermatis activity. Future in vivo experiments are needed to elucidate the safety and therapeutic potential of these combinations.RésuméMalassezia pachydermatis est un agent opportuniste important de la dermatite et de l'otite chez le chien. M. pachydermatis est généralement traité au moyen de traitements topiques utilisant une combinaison d'agents antifongiques, antimicrobiens et anti-inflammatoires. Nous avons étudié les activités in vitro du carvacrol (CRV), du cinnamaldehyde (CIN) et du thymol (THY) ...
Source: Journal of Medical Mycology - August 20, 2019 Category: Biology Source Type: research
Candidemia in a Portuguese tertiary care hospital: Analysis of a 2-year period
Conclusion: The distribution of Candida species has changed in recent years, with an increase in the proportion of C. albicans and C. glabrata. Rapid diagnostic tests, empiric antifungal therapy and source control are essential to improve the prognosis of patients with candidemia. More multicentric prospective studies are needed to evaluate the association of mortality with the timing of antifungal therapy or the EQUAL Candida Score. (Source: Journal of Medical Mycology)
Source: Journal of Medical Mycology - August 15, 2019 Category: Biology Source Type: research
Antifungal susceptibility and virulence factors of Candida spp. isolated from blood cultures
This study aims to investigate antifungal drug susceptibility, enzymatic activity and biofilm formation of the Candida spp. isolated from blood cultures. In 2016, a total of 84 clinical Candida isolates were analyzed for minimum inhibitory concentration (MIC) against fluconazole and amphotericin B by agar diffusion E-test (E strips). Three enzymatic activity tests for phospholipase, proteinase and esterase were performed by using egg yolk agar, bovine serum albumin medium and Tween 80 opacity medium, respectively. Biofilm formation was determined by crystal violet staining. To describing the various Candida distributions c...
Source: Journal of Medical Mycology - August 7, 2019 Category: Biology Source Type: research
Insights on the anticandidal activity of non-antifungal drugs
Publication date: Available online 27 July 2019Source: Journal de Mycologie MédicaleAuthor(s): Daniel Clemente de Moraes, Antonio Ferreira-PereiraAbstractThe search for new antifungal strategies to overcome Candida infections is essential and a matter of public health, due to the high mortality associated to candidiasis, the increasing incidence of resistance to antifungals and the limited number of drugs available for treatment. Several approaches have been exploited in order to develop new antifungal strategies, e.g. the use of natural products, vaccines, and the combination of an antifungal drug to a non-antifungal sub...
Source: Journal of Medical Mycology - July 28, 2019 Category: Biology Source Type: research
Fatal Cryptococcal Meningitis In A Child With Hyper-Immunoglobulin M Syndrome, With An Emphasis On The Agent
Publication date: Available online 26 July 2019Source: Journal de Mycologie MédicaleAuthor(s): Simone Mancini Liduário Suzuki, Fabrício Morelli, Melyssa Negri, Patricia Bonfim-Mendonça, Érika Seki Kioshima, Tania Salci, Morgana Ferreira Voidaleski, Vânia Aparecida Vicente, Terezinha SvidzinskiAbstractFollowing a fatal case of Cryptococcus neoformans meningitis in a child with X-linked hyper-immunoglobulin M syndrome (XHIGM), we evaluated the fungal isolate in an experimental infection in a mouse model with respect to microbiology, epidemiology, virulence and response to therapy. The minimum inhibitory concentrations ...
Source: Journal of Medical Mycology - July 27, 2019 Category: Biology Source Type: research
Retrograde signaling disruption influences ABC superfamily transporter, ergosterol and chitin levels along with biofilm formation in Candida albicans
Conclusion: Present study establishes a link between RTG signaling, drug efflux and biofilm formation and validates CaRTG3 as antifungal target. Intricate studies are needed to further understand and exploit this therapeutic opportunity. (Source: Journal of Medical Mycology)
Source: Journal of Medical Mycology - July 27, 2019 Category: Biology Source Type: research
