Issue Information
Journal of Cellular Biochemistry, Volume 120, Issue 1, Page 1-13, January 2019. (Source: Journal of Cellular Biochemistry)
Source: Journal of Cellular Biochemistry - November 21, 2018 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research
Optimizing methods for human testicular tissue cryopreservation and spermatogonial stem cell isolation
Cryopreservation of testicular tissue before cancer therapy for fertility preservation in prepubertal boys with cancer is of great interest in reproductive medicine. Isolation of spermatogonial stem cells (SSCs) from cryopreserved tissues would be a suitable cell source to re ‐establish spermatogenesis after cancer therapy. We herein establish optimized protocols for cryopreservation of human testicular tissue and isolation of SSCs from cryopreserved tissue. We developed a freezing protocol that provided high testicular cell viability and supported structural integrity and tubular epithelium coherence similar to fresh ti...
Source: Journal of Cellular Biochemistry - November 21, 2018 Category: Biochemistry Authors: Seyedeh ‐Faezeh Moraveji,
Fereshteh Esfandiari,
Mina Sharbatoghli,
Sara Taleahmad,
Saman Nikeghbalian,
Abdolhossein Shahverdi,
Hossein Baharvand Tags: RESEARCH ARTICLE Source Type: research
Esophageal cancer lymph node metastasis –associated gene signature optimizes overall survival prediction of esophageal cancer
In summary, the messenger RNA –based risk score model that we developed successfully predicts the survival of esophageal cancer. It is independent of clinical information and performs better than other clinical information for prognosis. AUC, area under the curve; CI, confidence interval. AbstractEsophageal cancer (EC) is characteristic of early regional lymph node metastasis (LNM) and most patients with metastasis have a poor prognosis. However, the current diagnostic techniques do not enable precise differentiation of EC LNM, prognostic stratification, and individual survival estimation. To identify potential molecular...
Source: Journal of Cellular Biochemistry - November 21, 2018 Category: Biochemistry Authors: Weiyang Cai,
Yanyan Li,
Bo Huang,
Changyuan Hu Tags: RESEARCH ARTICLE Source Type: research
Inhibitory role of lentivirus ‐mediated aquaporin‐4 gene silencing in the formation of glial scar in a rat model of traumatic brain injury
AbstractTraumatic brain injury (TBI), an acute degenerative pathology of the central nervous system, is a leading cause of death and disability. As the glial scar is a mechanical barrier to nerve regeneration, inhibitory molecules in the forming scar and methods to overcome them have suggested molecular modification strategies to allow neuronal growth and functional regeneration. Herein, we aim to investigate the effects of aquaporin ‐4 (AQP4) gene silencing on the glial scar formation after TBI by establishing rat models. After modeling, TBI rats were transfected with AQP4 small hairpin RNA [shRNA] (AQP4 gene silencing ...
Source: Journal of Cellular Biochemistry - November 21, 2018 Category: Biochemistry Authors: Ya ‐Xiong Yong,
Yu‐Ming Li,
Jia Lian,
Chuan‐Ming Luo,
De‐Xia Zhong,
Ke Han Tags: RESEARCH ARTICLE Source Type: research
CRNDE promotes cell tongue squamous cell carcinoma cell growth and invasion through suppressing miR ‐384
1.Colorectal neoplasia differentially expressed (CRNDE) expression was upregulated in tongue squamous cell carcinoma (TSCC) cell lines and tissues. Overexpression of CRNDE increased the TSCC cell proliferation, cell cycle, and cell invasion. CRNDE promoted TSCC cell proliferation and invasion through inhibiting miR ‐384 expression. AbstractTongue squamous cell carcinoma (TSCC) is the most common type of oral cancer and is an aggressive head and neck malignancy. Increasing studies have demonstrated that long noncoding RNAs (lncRNAs) play important roles in diverse biological cell processes, such as cell development, fate ...
Source: Journal of Cellular Biochemistry - November 21, 2018 Category: Biochemistry Authors: Yixiong Ren,
Wenting He,
Wenge Chen,
Chao Ma,
Yun Li,
Zhenyan Zhao,
Tao Gao,
Qianwei Ni,
Juan Chai,
Moyi Sun Tags: RESEARCH ARTICLE Source Type: research
Recent advances in endotoxin tolerance
AbstractEndotoxin tolerance is defined as a reduced capacity of a cell to respond endotoxin (lipopolysaccharide, LPS) challenge after an initial encounter with endotoxin in advance. The body becomes tolerant to subsequent challenge with a lethal dose of endotoxin and cytokines release and cell/tissue damage induced by inflammatory reaction are significantly reduced in the state of endotoxin tolerance. The main characteristics of endotoxin tolerance are downregulation of inflammatory mediators such as tumor necrosis factor α (TNF‐α), interleukin‐1β (IL‐1β), and C‐X‐C motif chemokine 10 (CXCL10) and upregulatio...
Source: Journal of Cellular Biochemistry - November 21, 2018 Category: Biochemistry Authors: Dan Liu,
Shousong Cao,
Yejiang Zhou,
Yuxia Xiong Tags: PROSPECTS Source Type: research
Whole transcriptome sequencing reveals biologically significant RNA markers and related regulating biological pathways in cardiomyocyte hypertrophy induced by high glucose
Our study gives new insights into cardiomyocyte hypertrophy and makes a great progress in comprehending its mechanism. AbstractCardiomyocyte hypertrophy is a physiological adaptation used in an attempt to augment or preserve cardiac function for short periods. Long ‐term cardiomyocyte hypertrophy often progresses to heart failure. Previous studies have presented comprehensive mechanisms underlying cardiomyocyte hypertrophy, such as signaling pathways, marker genes, and marker miRNAs or lncRNAs. However, the mechanism in RNA level is still unclear. In this st udy, we used the whole transcriptome technology on cardiomyocet...
Source: Journal of Cellular Biochemistry - November 21, 2018 Category: Biochemistry Authors: Zheying Meng,
Cui Chen,
Hongli Cao,
Jingyi Wang,
E Shen Tags: RESEARCH ARTICLE Source Type: research
Curcumin derivative L6H4 inhibits proliferation and invasion of gastric cancer cell line BGC ‐823
In this study, we investigated the anticancer activity of curcumin derivative L6H4 (curcumin L6H4) on gastric cancer cells. Inhibitory effects of curcumin L6H4 on gastric cancer cells (BGC‐823) were studied by the diphenyltetrazolium (MTT) assay, and cell apoptosis was detected by Annexin‐V/propidium iodide (PI) staining and then analyzed by flow cytometry. A mouse xenotransplant gastric tumor model was established to detect the role of curcumin L6H4 in vivo. The apoptosis‐related proteins p53, p21, Bax, and Bcl‐2 in BGC‐823 cells and mouse xenotransplant models treated with curcumin L6H4 were determined by Weste...
Source: Journal of Cellular Biochemistry - November 21, 2018 Category: Biochemistry Authors: Jianfeng Mu,
Xiaodong Wang,
Lihua Dong,
Pengda Sun Tags: RESEARCH ARTICLE Source Type: research
Evaluation on the effect of hydrogen sulfide on the NLRP3 signaling pathway and its involvement in the pathogenesis of atherosclerosis
ConclusionIn summary, the present finding suggested a possible linkage between H2S metabolism and AS through the H2S/CSE ‐TXNIP‐NLRP3‐IL‐18/IL‐1β‐nitric oxide (NO) signaling pathway. (Source: Journal of Cellular Biochemistry)
Source: Journal of Cellular Biochemistry - November 21, 2018 Category: Biochemistry Authors: Li ‐Ming Yue,
Ya‐Mei Gao,
Bao‐Hua Han Tags: RESEARCH ARTICLE Source Type: research
Dexmedetomidine alleviates cerebral ischemia ‐reperfusion injury in rats via inhibition of hypoxia‐inducible factor‐1α
Dexmedetomidine (Dex) can reduce apoptosis induced by cerebral ischemia ‐reperfusion (I/R). Increased expression of hypoxia‐inducible factor‐1α (HIF‐1α) was found in brain tissue of I/R rats. Inhibition of HIF‐1α reduces apoptosis induced by I/R. AbstractDexmedetomidine (Dex) was reported to reduce ischemia ‐reperfusion (I/R) injury in kidney and brain tissues. Thus, we aimed to study the role and mechanism of Dex in cerebral I/R injury by inhibiting hypoxia‐inducible factor‐1α (HIF‐1α) and apoptosis. First, I/R injury models were established. Six groups were assigned after different treatmen ts: sha...
Source: Journal of Cellular Biochemistry - November 19, 2018 Category: Biochemistry Authors: Yuan ‐Qing Wang,
Yu‐Feng Tang,
Ming‐Kun Yang,
Xi‐Zhao Huang Tags: RESEARCH ARTICLE Source Type: research
LncRNA FOXD2 ‐AS1 accelerates the papillary thyroid cancer progression through regulating the miR‐485‐5p/KLK7 axis
This study aims to explore the biological role and mechanism of FOXD2‐AS1 in PTC. At first, the expression of FOXD2‐ AS1 was examined in PTC tissues and cell lines with quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). FOXD2‐AS1 was found to observably upregulated in PTC tissues and cell lines. Kaplan‐Meier survival analysis revealed that high expression of FOXD2‐AS1 was closely cor related with the unfavorable prognosis of patients with PTC. Based on the TCGA data set, KLK7 was overexpressed in PTC tumor samples. Our experimental data further validated the upregulation of KLK7 in PTC ti...
Source: Journal of Cellular Biochemistry - November 19, 2018 Category: Biochemistry Authors: Yayuan Zhang,
Jintao Hu,
Wenbing Zhou,
Hengyuan Gao Tags: RESEARCH ARTICLE Source Type: research
TMEM106A inhibits cell proliferation, migration, and induces apoptosis of lung cancer cells
In this study, we evaluated the expression profile of TMEM106A in NSCLC tissues and cell line, and explored the roles of TMEM106A in NSCLC cell lines. Our results showed that TMEM106A expression was significantly decreased in human NSCLC tiss ues. In vitro assays showed that TMEM106A expression in NSCLC cell lines was much lower than that in the bronchial epithelial cell line. Besides, overexpression of TMEM106A reduced cell proliferation, migration, and invasion, while induced cell apoptosis in NSCLC cells. TMEM106A overexpression repre ssed epithelial‐mesenchymal transition (EMT), which was illustrated by increased E...
Source: Journal of Cellular Biochemistry - November 19, 2018 Category: Biochemistry Authors: Juncai Liu,
Hongjing Zhu Tags: RESEARCH ARTICLE Source Type: research
Oxymatrine ameliorates diabetes ‐induced aortic endothelial dysfunction via the regulation of eNOS and NOX4
ConclusionThe OMT treatment ameliorates diabetic endothelial dysfunction through enhanced NO bioavailability by upregulating eNOS expression and downregulating expression of NOX4. (Source: Journal of Cellular Biochemistry)
Source: Journal of Cellular Biochemistry - November 19, 2018 Category: Biochemistry Authors: Lei Wang,
Xinliang Li,
Yanqi Zhang,
Yongpan Huang,
Yinzhuang Zhang,
Qilin Ma Tags: RESEARCH ARTICLE Source Type: research
Role of miR ‐142 in the pathogenesis of osteosarcoma and its potential as therapeutic approach
The current review aimed to have overview on miR ‐142 as a tumor suppressor in osteosarcoma (OS). Additionally, the genes involved in the disease, such as RAC1, HMAG1, MMP9, MMP2, and E‐cadherin, which have irregularities as a result of change in miR‐142 expression, and, thereby, result in increasing the proliferation, invasion, and metastas is of the tumor cells in OS will be discussed. AbstractOsteosarcoma (OS) is the most common primary malignant tumor of the bone with a strong tendency to early metastasis, and occurs in growing bones more commonly in children and adolescents. Considering the limited therapeutic m...
Source: Journal of Cellular Biochemistry - November 18, 2018 Category: Biochemistry Authors: Parastoo Shabani,
Sama Izadpanah,
Ali Aghebati ‐Maleki,
Elham Baghbani,
Amir Baghbanzadeh,
Ali Fotouhi,
Babak Bakhshinejad,
Leili Aghebati‐Maleki,
Behzad Baradaran Tags: VIEW POINTS Source Type: research
Silencing of ADAM33 restrains proliferation and induces apoptosis of airway smooth muscle cells in ovalbumin ‐induced asthma model
Silencing of a disintegrin and metalloprotease 33 (ADAM33) can decrease the proliferation and increase the apoptosis of airway smooth muscle cells in a rat model of allergic asthma, suggesting ADAM33 represents a potential investigative focus target aiding allergic asthma. AbstractA defibrinogen and metalloproteinase 33 (ADAM33) was reported to play an important role in asthma. Furthermore, ADAM33 may play a possible role in airway remodeling due to its high expression in myo ‐/fibroblasts, epithelium, as well as the airway smooth muscle cells (ASMCs). Thus, the study is supposed to investigate the effect of the downregu...
Source: Journal of Cellular Biochemistry - November 18, 2018 Category: Biochemistry Authors: Jing Zhou,
Wei Bai,
Qin Liu,
Jian Cui,
Wei Zhang Tags: RESEARCH ARTICLE Source Type: research
