LotA, a Legionella deubiquitinase, has dual catalytic activity and contributes to intracellular growth
Abstract
The intracellular bacterial pathogen Legionella pneumophila establishes the replicative niche as a result of the actions of a large array of effector proteins delivered via the Legionella type IV secretion system (T4SS). Many effector proteins are expected to be involved in biogenesis and regulation of the Legionella‐containing vacuole (LCV) that is highly decorated with ubiquitin. Here, we identified a Legionella deubiquitinase (DUB), designated LotA, by carrying out a genome analysis to find proteins resembling the eukaryotic ovarian tumor (OTU) superfamily of cysteine proteases. LotA exhibits a dual ability t...
Source: Cellular Microbiology - March 15, 2018 Category: Microbiology Authors: Tomoko Kubori, Tomoe Kitao, Hiroki Ando, Hiroki Nagai Tags: RESEARCH ARTICLE Source Type: research
Pleiotropic effects of the histone deacetylase Hos2 linked to H4 ‐K16 deacetylation, H3‐K56 acetylation and H2A‐S129 phosphorylation in Beauveria bassiana
Abstract
Histone acetyltransferases (HATs) and deacetylases (HDACs) maintain dynamics of lysine acetylation/deacetylation on histones and non‐histone substrates involved in gene regulation and cellular events. Hos2 is a class I HDAC that deacetylates unique histone H4‐K16 site in yeasts. Here, we report that orthologous Hos2 deacetylates H4‐K16 and is also involved in the acetylation of histone H3‐K56 and the phosphorylation of histone H2A‐S129 and cyclin‐dependent kinase 1 CDK1‐Y15 in Beauveria bassiana, a filamentous fungal insect pathogen. These site‐specific modifications are evidenced with hyperacetyla...
Source: Cellular Microbiology - March 15, 2018 Category: Microbiology Authors: Qing Cai, Sen ‐Miao Tong, Wei Shao, Sheng‐Hua Ying, Ming‐Guang Feng Tags: RESEARCH ARTICLE Source Type: research
A coiled ‐coil protein is required for coordination of karyokinesis and cytokinesis in Toxoplasma gondii
Cellular Microbiology, EarlyView. (Source: Cellular Microbiology)
Source: Cellular Microbiology - March 13, 2018 Category: Microbiology Source Type: research
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Cellular Microbiology, Ahead of Print. (Source: Cellular Microbiology)
Source: Cellular Microbiology - March 13, 2018 Category: Microbiology Source Type: research
Identification and characterization of a Theileria annulata proline rich microtubule and SH3 ‐domain interacting protein (TaMISHIP) that forms a complex with CLASP1, EB1 and CD2AP at the schizont surface
Abstract
Theileria annulata is an apicomplexan parasite that modifies the phenotype of its host cell completely, inducing uncontrolled proliferation, resistance to apoptosis and increased invasiveness. The infected cell thus resembles a cancer cell, and changes to various host cell signaling pathways accompany transformation. Most of the molecular mechanisms leading to Theileria‐induced immortalization of leukocytes remain unknown. The parasite dissolves the surrounding host cell membrane soon after invasion and starts interacting with host proteins, ensuring its propagation by stably associating with the host cell micro...
Source: Cellular Microbiology - March 9, 2018 Category: Microbiology Authors: Sandra Huber, Tulin Karagenc, Dominic Ritler, Sven Rottenberg, Kerry Woods Tags: RESEARCH ARTICLE Source Type: research
NK cells inhibit anti ‐Mycobacterium bovis BCG T cell responses and aggravate pulmonary inflammation in a direct lung infection mouse model
Cellular Microbiology, EarlyView. (Source: Cellular Microbiology)
Source: Cellular Microbiology - March 9, 2018 Category: Microbiology Source Type: research
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Cellular Microbiology, Ahead of Print. (Source: Cellular Microbiology)
Source: Cellular Microbiology - March 9, 2018 Category: Microbiology Source Type: research
NK cells inhibit anti ‐Mycobacterium bovis BCG T cell responses and aggravate pulmonary inflammation in a direct lung infection mouse model
Abstract
Tuberculosis remains a threat to public health. The major problem for curing this disease is latent infection, of which the underlying mechanisms are still not fully understood. Previous studies indicate that natural killer (NK) cells do not play a role in inhibiting the growth of Mycobacterium tuberculosis in the lung, and recent studies have revealed that NK cells regulate the adaptive immunity during mycobacterial infection. By using a mouse model of direct lung infection with Mycobacterium bovis bacillus Calmette‐Guerin (BCG), we found that the presence of NK cells postponed the priming and activation of T c...
Source: Cellular Microbiology - March 9, 2018 Category: Microbiology Authors: Dongfang Wang, Xiuling Gu, Xiaoman Liu, Songtao Wei, Bin Wang, Min Fang Tags: RESEARCH ARTICLE Source Type: research
Exposure of helices α4 and α5 is required for insecticidal activity of Cry2Ab by promoting assembly of a prepore oligomeric structure
Cellular Microbiology, EarlyView. (Source: Cellular Microbiology)
Source: Cellular Microbiology - March 7, 2018 Category: Microbiology Source Type: research
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Cellular Microbiology, Ahead of Print. (Source: Cellular Microbiology)
Source: Cellular Microbiology - March 7, 2018 Category: Microbiology Source Type: research
Exposure of helices α4 and α5 is required for insecticidal activity of Cry2Ab by promoting assembly of a prepore oligomeric structure
Abstract
Cry2Ab, a pore‐forming toxin derived from Bacillus thuringiensis, is widely used as a bio‐insecticide to control lepidopteran pests around the world. A previous study revealed that proteolytic activation of Cry2Ab by Plutella xylostella midgut juice was essential for its insecticidal activity against P. xylostella, although the exact molecular mechanism remained unknown. Here, we demonstrated for the first time that proteolysis of Cry2Ab uncovered an active region (the helices α4 and α5 in Domain I), which was required for the mode of action of Cry2Ab. Either the masking or the removal of helices α4 and α...
Source: Cellular Microbiology - March 7, 2018 Category: Microbiology Authors: Lian Xu, Zhi ‐Zhen Pan, Jing Zhang, Li‐Yang Niu, Jie Li, Zheng Chen, Bo Liu, Yu‐Jing Zhu, Qing‐Xi Chen Tags: RESEARCH ARTICLE Source Type: research
Issue Information
Cellular Microbiology,Volume 20, Issue 4, April 2018. (Source: Cellular Microbiology)
Source: Cellular Microbiology - March 6, 2018 Category: Microbiology Source Type: research
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Cellular Microbiology,Volume 20, Issue 4, April 2018. (Source: Cellular Microbiology)
Source: Cellular Microbiology - March 6, 2018 Category: Microbiology Source Type: research
Issue Information
No abstract is available for this article. (Source: Cellular Microbiology)
Source: Cellular Microbiology - March 6, 2018 Category: Microbiology Tags: ISSUE INFORMATION Source Type: research
Interplay between Porphyromonas gingivalis and EGF signaling in the regulation of CXCL14
In this study, we investigated the inflammatory response of oral epithelial cells to P. gingivalis. The cells responded by upregulating the expression of the orphan chemokine CXCL14. The stimulation of CXCL14 expression was largely triggered by the gingipain proteases, and was dependent on the host protease‐activated receptor PAR‐3. Significantly, CXCL14 expression was transcriptionally repressed in response to EGF‐induced activation of the MEK‐ERK1/2 pathway. P. gingivalis overcomes the repression of CXCL14 via the gingipain protease‐mediated degradation of EGF. Therefore, P. gingivalis not only directly stimula...
Source: Cellular Microbiology - March 1, 2018 Category: Microbiology Authors: Jiamin Aw, Glen M. Scholz, Noorjahan Laila Huq, Jennifer Huynh, Neil M. O ’Brien‐Simpson, Eric C. Reynolds Tags: RESEARCH ARTICLE Source Type: research
