Yeast fermentation of sugarcane for ethanol production: Can it be monitored by using in situ microscopy?
ABSTRACT This paper addresses some key issues related to the automation of fermentation process analysis in the context of industrial-scale ethanol production from sugarcane substrates. As the current methods for the determination of cell density and viability are time consuming and laborious, high resolution in situ microscopy (0.5 µm) is proposed as a promising alternative. Laboratory-scale experiments presented here show that this imaging technique allows automatic, on-line, and real-time monitoring of yeast cells suspended in sugarcane molasses used in the ethanol industry. In particular, the feasibility of cell conce...
Source: Brazilian Journal of Chemical Engineering - February 15, 2018 Category: Chemistry Source Type: research

Pretreatments and enzymatic hydrolysis of sugarcane bagasse aiming at the enhancement of the yield of glucose and xylose
ABSTRACT This work studied the enzymatic hydrolysis of sugarcane bagasse aiming at the production of glucose and xylose. The bagasse was subjected to two different pretreatments: combined acid and alkalinepretreatment and hydrogen peroxidepretreatment. The enzymatic hydrolysis was optimized and a kinetic study was carried out in a stirred tank reactor (STR) in batch mode. Optimal conditions were obtained by subjecting the bagasse to the hydrogen peroxide pretreatment followed by enzymatic hydrolysis. The addition of xylanases to the enzymatic mixture improved the production of fermentable sugars by 48%. (Source: Brazilian ...
Source: Brazilian Journal of Chemical Engineering - February 15, 2018 Category: Chemistry Source Type: research

Fermentation of hexoses and pentoses from hydrolyzed soybean hull into ethanol and xylitol by Candida guilliermondii BL 13
Abstract This work investigated the ability of a recently isolated strain of Candida guilliermondii to convert hexoses and pentoses obtained from acid-enzymatic soybean hull hydrolysates into ethanol and, in smaller amounts, into xylitol. Operational conditions and media formulation were optimized concerning ethanol production using experimental designs (Plackett-Burman and Central Composite Design). Results showed that C. guilliermondii BL 13 was capable of growing in non-supplemented, non-detoxified biomass hydrolysates, and the best culture conditions were determined to be 28 °C, pH 5.0, and 109 CFU mL-1 of inoculum si...
Source: Brazilian Journal of Chemical Engineering - February 15, 2018 Category: Chemistry Source Type: research

Liquid-liquid extraction equilibrium for pyruvic acid recovery: experimental data and modeling
ABSTRACT Physical extraction studies of pyruvic acid from dilute aqueous solutions (0.025 kmol ·m-3 to 0.200 kmol·m-3) using several pure solvents (tri-n-butylphosphate (TBP), 1-decanol, 1-octanol, toluene, Methyl-isobutylketone (MIBK), and n-heptane) and their binary mixtures are reported in this work. All the batch extraction experiments were carried out at isothermal conditions (T=303±1 K) and the results are reported in terms of distribution coefficient (K D). The results obtained by the equilibrium studies of the different extraction system were correlated with solvatochromic parameters of the solvents. A linear so...
Source: Brazilian Journal of Chemical Engineering - February 5, 2018 Category: Chemistry Source Type: research

Liquid-Liquid Equilibrium Data, Viscosities, Densities, Conductivities, and Refractive Indexes of Poly (vinyl pyrrolidone)+ tri-sodium Citrate + Urea Aqueous Two-Phase Systems at Different pH
Abstract The present study investigated liquid-liquid equilibria of polyvinylpyrrolidone (PVP) K30 + tri-sodium citrate + urea (5% and 10% mass) aqueous two-phase systems at 25 °C and pH values of 6.2, 7.4, and 10.0. Results showed that the binodal was displaced toward higher concentrations as the urea concentration increased. The effect of pH, polymer/salt (w/w) ratio, urea concentration, tie line length, and slope of tie line on the partition behavior of urea was examin ed. The viscosity, density, electrical conductivity, and refractive index of PVP (K30) + tri-sodium citrate + urea + water two-phase systems were measur...
Source: Brazilian Journal of Chemical Engineering - February 5, 2018 Category: Chemistry Source Type: research