Entamoeba histolytica: identification of thioredoxin-targeted proteins and analysis of serine acetyltransferase-1 as a prototype example
Entamoeba histolytica, the causative agent of amoebiasis, possesses the dithiol-containing redox proteins thioredoxin (Trx) and thioredoxin reductase (TrxR). Both proteins were found to be covalently modified and inactivated by metronidazole, a 5-nitroimidazole drug that is commonly used to treat infections with microaerophilic protozoan parasites in humans. Currently, very little is known about enzymes and other proteins participating in the thioredoxin-dependent redox network of the parasite that could be indirectly affected by metronidazole treatment. Based on the disulphide/dithiol-exchange mechanism we constructed an ...
Source: BJ Energy - February 11, 2013 Category: Biochemistry Authors: S Schlosser, D Leitsch, M Duchêne Tags: BJ Energy Source Type: research
Nitrogen monoxide inhibits heme synthesis in mouse reticulocytes
Anemia of infection (AI) often manifests in patients with chronic immune
activation due to cancer, chronic infections, autoimmune disorders, rheumatoid
arthritis and other diseases. The pathogenesis of AI is complex and involves
cytokine-mediated inhibition of erythropoeisis, insufficient erythropoietin production
and diminished sensitivity of erythroid progenitors to this hormone, and retention of
iron in hemoglobin-processing macrophages. Nitric oxide (NO) is a gaseous
molecule produced by activated macrophages that has been identified as having
numerous effects on iron metabolism. Here we explore the possibility that NO...
Source: BJ Energy - January 30, 2013 Category: Biochemistry Authors: M R Mikhael, S Soe-Lin, S S. Apte, P Ponka Tags: BJ Metabolism Source Type: research
