siRNA delivery into cultured primary human myoblasts – optimization of electroporation parameters and theoretical analysis
In this study, we optimized the electroporation protocol for introduction of short interfering ribonucleic acid (siRNA) against messenger RNA for Hypoxia Inducible Factor 1α (HIF‐1α) into cultured primary human myoblasts. We established optimal pulsing protocol for siRNA electro transfection, and theoretically analyzed the effect of electric field and pulse duration on silencing efficiency and electrophoretic displacement of siRNA. Silencing of HIF‐1α was determined with quantitative polymerase chain reaction and Western Blot. The most efficient silencing (71% knockdown) was achieved with 8 × 2 ms pulses, E...
Source: Bioelectromagnetics - September 21, 2015 Category: Radiology Authors: Jasna Lojk, Katarina Mis, Sergej Pirkmajer, Mojca Pavlin Tags: Research Article Source Type: research
Does electromagnetic hypersensitivity originate from nocebo responses? Indications from a qualitative study
Idiopathic Environmental Intolerance attributed to Electromagnetic Fields (IEI‐EMF) is a condition in which symptoms are attributed to electromagnetic field (EMF) exposure. As electro‐hypersensitive (EHS) people have repeatedly been observed, during provocation trials, to report symptoms following perceived rather than actual exposure, the hypothesis has been put forward that IEI‐EMF originates from psychological mechanisms, especially nocebo responses. This paper examines this hypothesis, using data from a qualitative study aimed at understanding how EHS people come to regard themselves as such. Forty self‐diagnos...
Source: Bioelectromagnetics - September 1, 2015 Category: Radiology Authors: Maël Dieudonné Tags: Research Article Source Type: research
Single‐pulsed electromagnetic field therapy increases osteogenic differentiation through Wnt signaling pathway and sclerostin downregulation
This study enhances our understanding of cellular mechanisms to support the previous finding and may provide new insight for clinical applications. Bioelectromagnetics. 2015;9999:1–12. © 2015 Wiley Periodicals, Inc. (Source: Bioelectromagnetics)
Source: Bioelectromagnetics - September 1, 2015 Category: Radiology Authors: Chih‐Chun Lin, Ru‐Wei Lin, Chih‐Wei Chang, Gwo‐Jaw Wang, Kuo‐An Lai Tags: Research Article Source Type: research
SAR compliance assessment of PMR 446 and FRS walkie‐talkies
This study investigates compliance of walkie‐talkies to exposure guidelines established by the International Commission on Non‐Ionizing Radiation Protection and the Federal Communications Committee. The generic walkie‐talkie consisted of a helical antenna and a ground plane and was derived by reverse engineering of a commercial walkie‐talkie. Measured and simulated values of antenna characteristics and electromagnetic near fields of the generic walkie‐talkie were within 2% and 8%, respectively. We also validated normalized electromagnetic near fields of the generic walkie‐talkie against a commercial device and ...
Source: Bioelectromagnetics - September 1, 2015 Category: Radiology Authors: Günter Vermeeren, Wout Joseph, Luc Martens Tags: Research Article Source Type: research
Magnetic field effects on bistability and bifurcation phenomena in lipid peroxidation
A simple model based on physically evident assumptions was constructed that may predict the strong effect of low intensity magnetic fields (MFs) on living systems. As a model system, we studied MF effect on lipid peroxidation reaction under steady state conditions. It was assumed that external MF affects rate constants of radical reactions taking place in elementary stages of lipid peroxidation reaction. It was shown that under certain conditions, low intensity MF may provoke a bifurcation transition in the system followed by a significant change in its characteristics. This means that, in principle, an external MF may pla...
Source: Bioelectromagnetics - September 1, 2015 Category: Radiology Authors: Alexey Kipriyanov, Alexander Doktorov, Peter Purtov Tags: Research Article Source Type: research
Opinion on potential health effects of exposure to electromagnetic fields
In January 2015, the Scientific Committee on Emerging and Newly Identified Health Risks (SCENIHR) published its final opinion on “Potential health effects of exposure to electromagnetic fields.” The purpose of this document was to update previous SCENIHR opinions in the light of recently available information since then, and to give special consideration to areas that had not been dealt with in the previous opinions or in which important knowledge gaps had been identified. Bioelectromagnetics. 2015;9999:1–5. © 2015 Wiley Periodicals, Inc. (Source: Bioelectromagnetics)
Source: Bioelectromagnetics - July 16, 2015 Category: Radiology Tags: Letter to the Editor Source Type: research
Editorial: Introducing Dr. Frank S. Prato
(Source: Bioelectromagnetics)
Source: Bioelectromagnetics - June 10, 2015 Category: Radiology Authors: David R. Black Tags: Editorial Source Type: research
Non‐thermal extremely low frequency magnetic field effects on opioid related behaviors: Snails to humans, mechanisms to therapy
In 1984, it was initially discovered in mice that an extremely low frequency magnetic field (ELF‐MF) could attenuate opiate induced analgesia. In the past 30 years, we defined some of ELF‐MF exposure and subject state conditions that can both increase and decrease nociception in snails and mice and can induce analgesia in humans. In our search for mechanisms and our desire to translate our findings to the treatment of chronic pain in humans, we pioneered the use of electroencephalography and magnetic resonance imaging to monitor effects during exposure. We have contributed to an understanding of the phenomena but a con...
Source: Bioelectromagnetics - May 11, 2015 Category: Radiology Authors: Frank S. Prato Tags: Research Article Source Type: research
Effect of extremely low frequency magnetic fields on cell proliferation and gene expression
In this study, we investigated biological effects of ELF‐MF on MCF10A, MCF7, Jurkat, and NIH3T3 cell lines. ELF‐MF with a magnetic flux density of 1 mT at 60 Hz was employed to stimulate cells for 4 or 16 h, after which the effects of ELF‐MF on cell proliferation, cell death, cell viability, and DNA synthesis rates were assessed. Whereas Jurkat and NIH3T3 cells showed no consistent variation in cell number, cell viability, and DNA synthesis rate, MCF10A and MCF7 cells showed consistent and significant decreases in cell number, cell viability, and DNA synthesis rates. However, there was no effect of ELF‐MF on ...
Source: Bioelectromagnetics - May 1, 2015 Category: Radiology Authors: Hyung Chul Lee, Mi‐Na Hong, Seung Hee Jung, Bong Cho Kim, Young Ju Suh, Young‐Gyu Ko, Yun‐Sil Lee, Byeong‐Yoon Lee, Yeun‐Gyu Cho, Sung‐Ho Myung, Jae‐Seon Lee Tags: Research Article Source Type: research
Using software‐modified smartphones to validate self‐reported mobile phone use in young people: A pilot study
A newly developed smartphone application was piloted to characterize and validate mobile phone use in young people. Twenty‐six volunteers (mean age 17.3 years) from France, Spain, and the Netherlands used a software‐modified smartphone for 4 weeks; the application installed on the phone recorded number and duration of calls, data use, laterality, hands‐free device usage, and communication system used for both voice calls and data transfer. Upon returning the phone, participants estimated their mobile phone use during those 4 weeks via an interviewer‐administered questionnaire. Results indicated that participants on...
Source: Bioelectromagnetics - May 1, 2015 Category: Radiology Authors: Geertje Goedhart, Martine Vrijheid, Joe Wiart, Martine Hours, Hans Kromhout, Elisabeth Cardis, Chelsea Eastman Langer, Patricia de Llobet Viladoms, Amelie Massardier‐Pilonchery, Roel Vermeulen Tags: Letter to the Editor Source Type: research
Millimeter waves as a source of selective heating of skin
This study demonstrates that 20–100 GHz range can be used for spatially‐accurate focusing of heating inside the skin achieved by varying frequency and exposure beam size, as well as by enforcing air convection. The latter is also used to reduce overheating of skin surface. Heating at different skin depths depending on these parameters is investigated in detail using the hybrid bio‐heat equation. In particular, it is shown that decreasing frequency and/or increasing exposure beam size at forced airflow result in elevation of heating of deeper layers of tissue and decrease of skin surface temperature. Changes of wate...
Source: Bioelectromagnetics - May 1, 2015 Category: Radiology Authors: Maxim Zhadobov, Stanislav I. Alekseev, Yves Le Dréan, Ronan Sauleau, Evgeny E. Fesenko Tags: Research Article Source Type: research
The 2014 Most Influential Bioelectromagnetics Journal Paper by Citation Award
(Source: Bioelectromagnetics)
Source: Bioelectromagnetics - May 1, 2015 Category: Radiology Authors: James C. Lin Tags: Editorial Source Type: research
A novel method to assess human population exposure induced by a wireless cellular network
This paper presents a new metric to evaluate electromagnetic exposure induced by wireless cellular networks. This metric takes into account the exposure induced by base station antennas as well as exposure induced by wireless devices to evaluate average global exposure of the population in a specific geographical area. The paper first explains the concept and gives the formulation of the Exposure Index (EI). Then, the EI computation is illustrated through simple phone call scenarios (indoor office, in train) and a complete macro urban data long‐term evolution scenario showing how, based on simulations, radio‐planning p...
Source: Bioelectromagnetics - May 1, 2015 Category: Radiology Authors: Nadège Varsier, David Plets, Yoann Corre, Günter Vermeeren, Wout Joseph, Sam Aerts, Luc Martens, Joe Wiart Tags: Research Article Source Type: research
Calcium signalling in human neutrophil cell lines is not affected by low‐frequency electromagnetic fields
This study aimed to investigate the potential effect of LF EMF exposure on calcium signalling in neutrophils. In neutrophilic granulocytes, activation of G‐protein coupled receptors leads to efflux of calcium from calcium stores and influx of extracellular calcium via specialised calcium channels. The cytoplasmic rise of calcium induces cytoskeleton rearrangements, modified gene expression patterns, and cell migration. If LF EMF modulates intracellular calcium signalling, this will influence cellular behaviour and may eventually lead to health problems. We found that calcium mobilisation upon chemotactic stimulation was ...
Source: Bioelectromagnetics - May 1, 2015 Category: Radiology Authors: Lieke A. Golbach, John G.M. Philippi, Jan J.M. Cuppen, Huub F.J. Savelkoul, B.M. Lidy Verburg‐van Kemenade Tags: Research Article Source Type: research
Suppression of Arabidopsis flowering by near‐null magnetic field is affected by light
We previously reported that a near‐null magnetic field suppressed Arabidopsis flowering in white light, which might be related to the function modification of cryptochrome (CRY). To further demonstrate that the effect of near‐null magnetic field on Arabidopsis flowering is associated with CRY, Arabidopsis wild type and CRY mutant plants were grown in the near‐null magnetic field under blue or red light with different light cycle and photosynthetic photon flux density. We found that Arabidopsis flowering was significantly suppressed by near‐null magnetic field in blue light with lower intensity (10 µmol/m2/s) and...
Source: Bioelectromagnetics - May 1, 2015 Category: Radiology Authors: Chunxiao Xu, Yue Li, Yang Yu, Yuxia Zhang, Shufeng Wei Tags: Brief Communication Source Type: research
