A novel Ca2+-binding protein influences photosynthetic electron transport in Anabaena sp. PCC 7120
Publication date: Available online 27 April 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Julia Walter, Khaled A. Selim, Francisco Leganés, Francisca Fernández-Piñas, Ute C. Vothknecht, Karl Forchhammer, Eva-Mari Aro, Peter J. GollanAbstractCa2+ is a potent signalling molecule that regulates many cellular processes. In cyanobacteria, Ca2+ has been linked to cell growth, stress response and photosynthesis, and to the development of specialist heterocyst cells in certain nitrogen-fixing species. Despite this, the pathways of Ca2+ signal transduction in cyanobacteria are poorly understood, and v...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - April 27, 2019 Category: Biochemistry Source Type: research

Photosynthetic regulation under fluctuating light in young and mature leaves of the CAM plant Bryophyllum pinnatum
In this study, we measured PSI redox state and the electrochromic shift signal in the mature and young leaves of a CAM species Bryophyllum pinnatum. The mature leaves showed stronger capacity for photo-reduction of O2 mediated by the alternative electron flow (probably the water-water cycle) when compared with the young leaves. After an increase in light intensity, both the mature and young leaves showed insufficient proton gradient (ΔpH) across the thylakoid membranes within the first seconds. Meanwhile, PSI was highly oxidized in the mature leaves but was in a more reduced state in the young leaves. Furthermore, young l...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - April 26, 2019 Category: Biochemistry Source Type: research

Different roles for ApcD and ApcF in Synechococcus elongatus and Synechocystis sp. PCC 6803 phycobilisomes
Publication date: Available online 25 April 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Pablo I. Calzadilla, Fernando Muzzopappa, Pierre Sétif, Diana KirilovskyAbstractThe phycobilisome, the cyanobacterial light harvesting complex, is a huge phycobiliprotein containing extramembrane complex, formed by a core from which rods radiate. The phycobilisome has evolved to efficiently absorb sun energy and transfer it to the photosystems via the last energy acceptors of the phycobilisome, ApcD and ApcE. ApcF also affects energy transfer by interacting with ApcE. In this work we studied the role of Ap...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - April 26, 2019 Category: Biochemistry Source Type: research

Ammonia as a substrate-water analogue in photosynthetic water oxidation: Influence on activation barrier of the O2-formation step
Publication date: Available online 26 April 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Ricardo Assunção, Ivelina Zaharieva, Holger DauAbstractInformation on binding and rearrangement of pivotal water molecules could support understanding of light-driven water oxidation at the catalytic Mn4CaO5 cluster of photosystem II (PSII). To address this point, the binding of ammonia (NH3)—a possible substrate-water analogue—has been investigated and discussed in the context of putative reaction mechanisms. By time-resolved detection of O2 formation after light-flash excitation, we discriminate thr...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - April 26, 2019 Category: Biochemistry Source Type: research

Fourier transform visible and infrared difference spectroscopy for the study of P700 in photosystem I from Fischerella thermalis PCC 7521 cells grown under white light and far-red light: Evidence that the A–1 cofactor is chlorophyll f
Publication date: Available online 13 April 2019Source: Biochimica et Biophysica Acta (BBA) - BioenergeticsAuthor(s): Gary Hastings, Hiroki Makita, Neva Agarwala, Leyla Rohani, Gaozhong Shen, Donald A. BryantAbstract(P700+ – P700) Fourier transform visible and infrared difference spectra (DS) have been obtained using photosystem I (PSI) complexes isolated from cells of Fischerella thermalis PCC 7521 grown under white light (WL) or far-red light (FRL). PSI from cells grown under FRL (FRL-PSI) contain ~8 chlorophyll f (Chl f) molecules (Shen et al., Photosynth. Res. Jan. 2019). Both the visible and infrared DS indicate tha...
Source: Biochimica et Biophysica Acta (BBA) Bioenergetics - April 13, 2019 Category: Biochemistry Source Type: research