Abstract B32: Oncogenic role of snoRD93 in breast cancer cells
In this study, we compared the transcriptome profiles of human breast cancer cell lines, MCF-7 and MDA-MB-231, by using the next generation sequencing (NGS). MCF-7 cells were derived from the primary breast tumor, while MDA-MB-231 cells are highly metastatic. Our sequencing results showed that 13 snoRNAs was significantly overexpressed in MDA-MB-231 cells versus MCF-7 cells. In particular, we are interested in snoRD93, given its expression showing 27-time higher in MDA-MB-231 cells than MCF-7 cells. We further studied the oncogenic role of snoRD93 in MDA-MB-231 cells by using loss-of-function strategy. By employing cell vi...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Ma, R., Zhao, H., Patterson, D., Borchert, G., Xi, Y. Tags: Other Topics: Poster Presentations - Proffered Abstracts Source Type: research

Abstract B31: Sulindac inhibition of tumor cell transformation
This study is supported by the NIH/NCI R01 Grant (1R01CA192395) and the American Cancer Society Research Scholar Grant (RSG-13-265-01-RMC).Citation Format: Zhipin Liang, Xiangling Feng, Hong Chang, Bin Yi, Ruixia Ma, Yaguang Xi. Sulindac inhibition of tumor cell transformation. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Cancer Cell Cycle - Tumor Progression and Therapeutic Response; Feb 28-Mar 2, 2016; Orlando, FL. Philadelphia (PA): AACR; Mol Cancer Res 2016;14(11_Suppl):Abstract nr B31. (Source: Molecular Cancer Research)
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Liang, Z., Feng, X., Chang, H., Yi, B., Ma, R., Xi, Y. Tags: Other Topics: Poster Presentations - Proffered Abstracts Source Type: research

Abstract A31: Reprimo, a potential tumor suppressor gene TP53-dependent, modulates negatively cell migration and invasion in the MDA-MB-231 breast cancer cell line
Conclusion: Taken together, these data suggest that RPRM is involved in decreased cell migration and invasion in vitro, acting as a potential tumor suppressor gene in the MDA-MB-231 cell line.Citation Format: Kurt Buchegger, Tamara Viscarra, Carmen Gloria Ili, Ismael Riquelme, Pablo Letelier, Alejandro Corvalan, Priscilla Brebi, Tim Hui-Ming Huang, Juan Carlos Roa. Reprimo, a potential tumor suppressor gene TP53-dependent, modulates negatively cell migration and invasion in the MDA-MB-231 breast cancer cell line. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Cancer Cell Cycle - Tumor Progression and Th...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Buchegger, K., Viscarra, T., Ili, C. G., Riquelme, I., Letelier, P., Corvalan, A., Brebi, P., Huang, T. H.-M., Roa, J. C. Tags: Other Topics: Poster Presentations - Proffered Abstracts Source Type: research

Abstract B30: Resistance to Photodynamic Therapy in Non-Melanoma Skin Cancer Cells
Conclusion: The results indicate that it was possible to obtain HSC-1 cells two-fold resistant to PDT compared to their parental cells.Acknowledgments: Financial support by CONICYT. CONICYT-PCHA/Doctorado Nacional/2012-21120665, CORFO 09CN14-5960, Corfo N°12IDL2-18157, FONDECYT N° 11150802, FONDECYT N°11150622.Citation Format: Daniela Leon, Ramon Silva, Natalia Inada, Cristina Kurachi, Carmen Gloria Ili, Priscilla Brebi, Juan Carlos Roa. Resistance to Photodynamic Therapy in Non-Melanoma Skin Cancer Cells. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Cancer Cell Cycle - Tumor Progression a...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Leon, D., Silva, R., Inada, N., Kurachi, C., Ili, C. G., Brebi, P., Roa, J. C. Tags: Other Topics: Poster Presentations - Proffered Abstracts Source Type: research

Abstract A30: The effects of Berberis vulgaris on bacterial inhibition and the p53 gene in cancerous Caenorhabditis elegans
This study aims at finding the most effective natural antibacterial remedy. The disk diffusion method was used to compare the antibacterial properties of manuka honey, barberry, aloe vera, and colloidal silver. After a 48-hour incubation period, the zone of inhibition was measured for the following bacteria: Bacillus megaterium, Serratia marcescens, Pseudomonas fluorescens, Rhodospirillum rubrum, and Escherichia coli. Barberry was the most efficient natural product (p=0.02). Further tests were performed to determine whether barberry contained strong anticancer properties. Barberry was tested on Caenorhabditis elegans (C.el...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Bhatt, J. P. Tags: Other Topics: Poster Presentations - Proffered Abstracts Source Type: research

Abstract B29: ZNF516 a potential tumor suppressor gene candidate is implied in tumor progression in cervical cancer
Conclusions: There is a clear ZNF516 dysregulation in CC cell lines, and the inactivation mechanism seem to be methylation. Restored expression of ZNF516 in C-33A cell line modifies the tumor phenotype, decreasing cellular viability and colony formation. These results suggest that ZNF516 could be a TSG, and its inactivation promotes CC developing.Citation Format: Carmen Gloria Ili, Tamara Viscarra, Juan Carlos Araya, Jaime Lopez, Barbara Mora, Javier Retamal, Enrique Bellolio, Susana Aedo, Juan Carlos Roa, Priscilla Brebi. ZNF516 a potential tumor suppressor gene candidate is implied in tumor progression in cervical cancer...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Ili, C. G., Viscarra, T., Araya, J. C., Lopez, J., Mora, B., Retamal, J., Bellolio, E., Aedo, S., Roa, J. C., Brebi, P. Tags: Other Topics: Poster Presentations - Proffered Abstracts Source Type: research

Abstract B28: FKBP6 gene is involved in progression of cervical cancer
Conclusions: There is a FKBP6 dysregulation in CC cell lines, and the inactivation mechanism seems to be methylation. Restored expression of FKBP6 in SiHa cell line modifies the tumor phenotype, decreasing colony formation, but increasing cell viability. These results suggest that FKBP6 could be implied in cervical progression, but more studies are necessary to evaluate FKBP6 functions in cervical cancer.Acknowledgments: FONDECYT N°3130630, Project Corfo N° 09CN14-5960, Project Corfo N°12IDL2-18157. Project FONDECYT N° 11150802, Project FONDECYT N°1115062Citation Format: Carmen Gloria Ili, Tamara Viscar...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Ili, C. G., Viscarra, T., Araya, J. C., Lopez, J., Mora, B., Retamal, J., Aedo, S., Bellolio, E., Roa, J. C., Brebi, P. Tags: Other Topics: Poster Presentations - Proffered Abstracts Source Type: research

Abstract A28: Mechanistic study involving the combined antiproliferative effect of Etoricoxib- cyclooxygenase-2 inhibitor and cholecystokinin-2 receptor antagonist in human pancreatic cancer cells
Conclusion: The combined effect of Cyclooxygenase-2 inhibitor and Cholecystokinin-2 Receptor antagonists was calculated using Chaou Tatlay method. A combination index was calculated. As a class NSAIDs possess analgesic, antipyretic, anti-inflammatory, and there is persuasive evidence that COX-2 inhibitor suppress cancer cell proliferation owing to their role in apoptosis, compelling evidence suggest that COX-2 over-expression promotes whereas COX-2 inhibition prevents tumor initiation and promotions. NSAIDs and COX-2 selective inhibitors may have different effects on cancer may be stage dependent therefore a better underst...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Sikka, M. Tags: Managing G2/M Control: Poster Presentations - Proffered Abstracts Source Type: research

Abstract IA27: Perturbation of host cellular regulatory networks by human papillomaviruses
Human papillomaviruses (HPVs) are small viruses with ~8,000 bp double-stranded, circular genomes. There are over 200 HPV types and they all infect epithelial cells. While most HPV infections are subclinical others cause overt lesions. Most HPV-associated lesions are benign but some "high-risk" HPVs cause lesions that have a propensity for malignant progression and approximately 10% of all human cancers worldwide are caused by high-risk HPV infections. HPV-associated cancers generally represent non-productive infections, i.e. viral proteins are synthesized but no infectious virus is produced. HPV-associated cancers retain e...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Münger, K. Tags: Derailed by Infection: Viral-mediated Cell Cycle Dysfunction: Oral Presentations - Invited Abstracts Source Type: research

Abstract B27: Kinetochore-microtubule attachments as a precision therapy target
Glioblastoma multiforme (GBM) is an aggressive and refractory cancer with limited therapeutic strategies and poor survival. One challenge in treating this disease is the ability of single glioblastoma stem-like cells (GSCs) to initiate tumors. To expand the therapeutic repertoire for GBM we performed RNAi screening in GSCs and a proposed cell of origin, neural crest stem cells (NSCs). In these screens we identified multiple regulators of kinetochore-microtubule attachments as specifically required for GSC survival. Kinetochores are composed of hundreds of proteins that form dynamic attachments between microtubules and chro...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Herman, J. A., Paddison, P. J., DeLuca, J., Olson, J. Tags: Other Topics: Poster Presentations - Proffered Abstracts Source Type: research

Abstract A27: Cell cycle control and drugs targeting the bioenergetics of cancer
Differences in responsiveness to chemotherapeutics among patients may be associated with multiple cell types that can be found in tumors and the circulation. Recurrent or refractory disease may result from natural selection of cells in response to therapy. To design new drugs and make better use of existing drugs differences between diseased and disease causing cells in response to a specific drug requires investigation. The Warburg Effect is characteristic of the majority of cancer cell types both in tumors and the circulation. Drugs that target the underlying biochemistry of essential bioenergetic processes unique to can...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Shorr, R. Tags: Managing G2/M Control: Poster Presentations - Proffered Abstracts Source Type: research

Abstract B26: MiR-200 is involved in anti-invasive activity of sulindac in colon cancer
This study is supported by the NIH/NCI R01 Grant (1R01CA192395) and the American Cancer Society Research Scholar Grant (RSG-13-265-01-RMC).Citation Format: Hong Chang, Xiangling Feng, Ruixia Ma, Yaguang Xi. MiR-200 is involved in anti-invasive activity of sulindac in colon cancer. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Cancer Cell Cycle - Tumor Progression and Therapeutic Response; Feb 28-Mar 2, 2016; Orlando, FL. Philadelphia (PA): AACR; Mol Cancer Res 2016;14(11_Suppl):Abstract nr B26. (Source: Molecular Cancer Research)
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Chang, H., Feng, X., Ma, R., Xi, Y. Tags: Other Topics: Poster Presentations - Proffered Abstracts Source Type: research

Abstract B24: Ataxia-telangiectasia and Rad3-related (ATR) phosphorylation as a pharmacodynamic biomarker of ATR activation in solid tumor tissue models
Inhibitors of checkpoint kinases, such as ATR, Chk1 or Wee1, in combination with cytotoxic agents could enhance therapeutic efficacy compared to monotherapy, and these combination approaches are currently being extensively explored. The presence of replicative stress or deregulated S-phase in cancer has been recognized as a rationale for the use of ATR and Chk1 inhibitors with chemotherapy and efforts are underway to define genetic determinants that sensitize cancer cells to ATR inhibition. Pharmacodynamic (PD) biomarkers of drug activity are valuable tools in clinical trials using targeted agents to determine whether each...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Wilsker, D., Marrero, A. M., Dull, A., Pfister, T. D., Lawrence, S. M., Carter, J., Gottholm-Ahalt, M., Hollingshead, M., Doroshow, J., Parchment, R. E., Kinders, R. J. Tags: Replication Stress and DNA Damage Response: Poster Presentations - Proffered Abstracts Source Type: research

Abstract B23: The deubiquitinating enzyme USP37 stabilizes Chk1 to promote the cellular response to replication stress
Ubiquitin-mediated proteolysis is a key regulatory process in cell cycle progression. Recently, we identified mutual antagonism between the deubiquitinase USP37 as and the tumor suppressor APCCdh1 to regulate S-phase entry. Here we report that elevated USP37 expression correlates with cellular transformation. Depletion of USP37 leads to diminished cellular proliferation and loss of viability in tumor cells. USP37-depleted cells exhibit altered replication kinetics and increased levels of the DNA damage markers H2AX and 53BP1 as well as increased sensitivity to agents that induce replication stress. Underlying the increased...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Singh, M., Pal, D., Burrows, A. C., Messina, A., Dickson, A., Summers, M. K. Tags: Replication Stress and DNA Damage Response: Poster Presentations - Proffered Abstracts Source Type: research

Abstract B22: IT-141, a stabilized polymer micelle formulation, prolongs the pharmacodynamic effect of SN-38
IT-141 is a formulation of SN-38 encapsulated in an iron-stabilized polymer micelle. SN-38 is the active metabolite of irinotecan (CPT-11) which in combination with 5-FU and leucovorin is first-line FDA approved therapy for metastatic colorectal cancer. Although SN-38 is 1,000 times more potent than irinotecan alone, there is about 100-fold lower concentration of SN-38 in plasma from irinotecan. In the clinic only 2% to 10% of the administered dose of irinotecan is converted by carboxylesterases to SN-38 and there is great interpatient variability with toxicity. In vitro, IT-141 demonstrated nanomolar IC50s against a panel...
Source: Molecular Cancer Research - November 9, 2016 Category: Cancer & Oncology Authors: Sethuraman, J., Costich, T. L., Carie, A., Buley, T., Ellis, T., Semple, J. E., Vojkovsky, T., Sill, K., Bakewell, S. Tags: Replication Stress and DNA Damage Response: Poster Presentations - Proffered Abstracts Source Type: research