O-083 Non-invasive, label-free optical analysis to detect aneuploidy within the inner cell mass of the preimplantation embryo
AbstractStudy questionCan we separate between control and reversine-treated cells within the inner cell mass (ICM) of the mouse preimplantation embryo by using label-free and non-invasive hyperspectral microscopy?Summary answerHyperspectral microscopy is able to discern between control and reversine-treated cells using cellular autofluorescence in the complete absence of fluorescence tags.What is known alreadyEmbryo mosaicism (containing cells that are euploid (46 chromosomes) and aneuploid (deviation from the expected number of chromosomes)) affects up to 17.3% of human blastocyst embryos. Current diagnosis of aneuploidy ...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-079 Could ovarian reserve be affected after SARS-CoV-2 infection?
AbstractStudy questionIs there a variation in ovarian reserve in women who have passed the disease?Summary answerThe fact of having passed SARS-CoV-2 does not affect the ovarian reserve statusWhat is known alreadyDespite the overwhelming magnitude of this pandemic and its worldwide prevalence, information regarding the effects of the novel coronavirus on human reproduction are currently limited. As the assisted reproductive technology programs resumed operations, it was important to gather information regarding the status of individuals infected with the novel coronavirus, and to assess gametes and reproductive outcomes fo...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –757 The risk of aspirin and prednisone using in women with antithyroid antibodies undergoing assisted reproductive technology
In this study, likelihood of clinical pregnancy (P = 0.066) did not change significantly after therapy, and miscarriage rate (P = 0.042) increased after medical treatment.Limitations, reasons for cautionFirstly, Aspirin is just one representation of anticoagulation therapy, so additional consideration of low molecular heparin should also be considered. Secondly, further randomized controlled trials of aspirin and prednisone alone are needed.Wider implications of the findings: In this study, use of A+P showed no positive effect, and reduced cleavage rate and implantation rate, while increased miscarriage rate. So, t...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –756 Predictive factors influencing multiple live births in cumulative IVF cycles: retrospective analysis of over 265000 embryo transfer procedures from the national French registry
This study is limited in only providing a risk-benefit balance for multiples on the choice of transferring one or two embryos. Clinical data such as stimulation protocols and doses of gonadotropins were not considered in this evaluation.Wider implications of the findings: This study provides help to develop a strategy for the medical staff in the decision making for the number of embryos to be transferred. It may also serve as a patient ’s information aid and help to improve their chances of achieving a health singleton if pregnant.Trial registration numberNot applicable (Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –755 Perinatal outcomes following Day–4 embryo transfer compared to Day–2, Day–3 and Day–5 embryo transfer: an analysis of 56,346 singleton live births
AbstractStudy questionAre perinatal outcomes of singleton live births following Day –4 embryo transfer (ET) different to Day–2, Day–3 and Day–5 ET?Summary answerPerinatal outcomes of singleton live births following Day –4 ET are similar with those following Day–2, Day–3 and Day–5 ET.What is known alreadyThe morula represents a critical stage in preimplantation embryo development, but the usage of morula transfer on Day –4 has received little attention. Recent work from our group suggested that live birth rates following Day–4 ET appear higher than cleavage-stage ET, but lower than blastocyst ET. Therefo...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –754 A novel warmed device protecting the embryo transfer catheter is effective in preventing the cooling of embryos during the embryo transfer procedure
AbstractStudy questionCan cooling of embryos during the embryo transfer be alleviated with the use of a 37 °C temperature protective device covering the ET catheter?Summary answerCooling of embryos during embryo transfer can be effectively alleviated by using a 37 °C pre-warmed temperature protective device covering the ET catheter.What is known alreadyAn optimized physicochemical environment is crucial for maintenance of normal homeostasis, metabolism, and spindle stability to minimize stress on gametes and embryos. During preimplantation embryo development, epigenetic reprogramming occurs and environmental stress...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –753 A randomised controlled trial comparing expectant management versus intrauterine insemination in couples with unexplained subfertility and a poor prognosis for natural conception
AbstractStudy questionDoes 6 months expectant management reduces ongoing pregnancy rates compared to intrauterine insemination with ovarian stimulation (IUI-OS) in couples with unexplained subfertility?Summary answerIn couples with unexplained subfertility and a poor prognosis for natural conception, 6 months of expectant management decreases ongoing pregnancy rates as compared to IUI-OS.What is known alreadyIn couples with unexplained subfertility and a poor prognosis, IUI-OS is a first line treatment. We have previously shown that in couples with unexplained subfertility and a good prognosis for natural conception (>3...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –752 Embryo morphological grading across several IVF centers is not consistent but an interactive training is useful to improve its consistency
AbstractStudy questionAre the embryologists across several IVF clinics concordant when evaluating embryo morphology?Summary answerEmbryo morphological grading is sufficiently consistent among embryologists from the same center, while an interactive training was essential to improve its accuracy across several clinics.What is known alreadyEmbryo morphology, mostly at the blastocyst stage, is the strongest non-invasive embryological feature that associates with implantation potential. This association is confirmed also when euploid blastocysts are transferred. At present, several embryo grading schemes exist but is still unc...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –751 Immediate versus postponed frozen-thawed embryo transfer after IVF/ICSI: a systematic review and meta-analysis
AbstractStudy questionCan frozen embryo transfer (FET) be offered immediately after a stimulated IVF/ICSI cycle without compromising live birth rate (LBR)?Summary answerFET in the menstrual cycle immediately following the stimulated IVF/ICSI cycle was associated with a slightly higher LBR compared to standard postponed FET.What is known alreadyIt is standard clinical practice to postpone FET for at least one menstrual cycle following a failed fresh transfer or a freeze-all cycle. This practice is thought to minimize any possible residual negative effect of ovarian stimulation, with excessive steroid levels and multiple cor...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –342 Are blastulation and clinical pregnancy rates in women with endometriomas different than those without?
AbstractStudy questionDoes the presence of endometrioma during ovarian stimulation affect blastulation and clinical pregnancy rates (CPR)?Summary answerBlastulation rates were similar in women with endometrioma compared to women without. Likewise, CPR were comparable.What is known alreadyAlthough relationship of endometriosis and subfertility is well-established, its mechanism is still under investigation. Decreased oocyte quality, resulting from anatomical and/or inflammatory factors is one of the prominent culprits. Most studies regarding endometriosis and oocyte quality are highly heterogeneous and effect of endometrios...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –165 Using Artificial Intelligence to Classify Embryo Shape: An International Perspective
This study aims to evaluate the transferability and robustness of this model on blind test data from a different country (France).Study design, size, durationThe study was a retrospective cohort analysis in which 909 4-cell embryo images ( “tetrahedral”, n = 749; “non-tetrahedral”, n = 160) were collected from 3 clinics (2 Brazilian, 1 French). All embryos were captured at the central focal plane using Embryoscope™ time-lapse incubators. The training data consisted solely of embryo images captured in Brazil (586 tetr ahedral; 87 non-tetrahedral) and the test data consisted exclusively of embryo images cap...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –164 Mulitcentre derived time lapse algorithms developed using 6228 transferred embryos with known birth outcome incorporating novel morphological and morphokinetic markers
AbstractStudy questionCan incorporation of novel markers of morphology with known temporal events successfully rank embryos to enable prediction of propensity for live birth?Summary answerIncorporation of variables for trophectoderm and morula grading demonstrably enhanced the model to rank embryos in order of potential for live birth.What is known alreadyModels built using morphokinetic markers of development are widely used to rank embryos within a cohort. Such models include defined temporal parameters which are closely related to morphological grade. However, morphological grading by an embryologist is subjective and i...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –163 The presence of trophectodermal vesicles on expanded ICSI-derived blastocysts is a good predictor of implantation
This study included only transfers of the ICSI-derived fully expanded blastocysts with top-graded inner cell mass and trophectoderm. AH was performed using laser Saturn 3. The primary outcome was the implantation rate. Statistical analysis was performed using Pearson ’s chi-square test and likelihood ratio test. Preimplantation genetic testing for aneuploidy (PGT-A) was performed by next-generation sequencing.Main results and the role of chanceThe number of cases in groups 1,2 and 3 was 133, 49, and 295, respectively. The average age in the groups was about 32.5 and did not differ between groups. The implantation rate in...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –162 Laser-assisted collapse of blastocysts prior to vitrification improves clinical outcomes
AbstractStudy questionWhat is the effect of artificial laser-assisted collapse before vitrification on pregnancy and implantation rates after transfer of vitrified-warmed blastocysts?Summary answerThe artificial shrinkage by laser-induced collapse before vitrification significantly increased the implantation and clinical pregnancy rates after single thawed embryo transfer.What is known alreadyFreeze all, cycle segmentation and, in general, single embryo transfer (SET) strategies (for example trophectoderm biopsy-based aneuploidy testing) have targeted blastocysts vitrification as the best option for reproductive practice w...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –161 Is enough the staff in your lab?
AbstractStudy questionMust be all the activity made in in vitro fertilization (IVF) laboratories keep in mind to size its staff?Summary answerTo create a staff calculator based on number of cycles carry on, assisted reproduction techniques, quality controls, administration management, weekend duties, labour regulations and holidays.What is known alreadyIn a bibliographic search about staff in human reproduction labs there is no mention about de number of embryologists recommended for every cycle done. Only that it will be according to the workload. Other guidelines establish that every embryologist could assume 150 IVF cyc...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
