P –104 Assessment of sperm motility according to WHO classification using convolutional neural networks
AbstractStudy questionHow does convolutional neural network (CNN)-predicted sperm motility correlate with manual assessment according to the WHO guidelines.Summary answerCNN predicts sperm motility comparable to reference laboratories in the ESHRE-SIGA External Quality Assessment Programme for Semen Analysis.What is known alreadyManual sperm motility assessment according to WHO guidelines is regarded as the gold standard. To obtain reliable and reproducible results, comprehensive training is essential as well as running internal and external quality control. Prediction based on artificial intelligence can potentially trans...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –103 Novel sperm preparation techniques compared with conventional preparation method
In this study, each sample was divided into 4 groups, one part for preparing by Microfluidic method, one of them for preparing by Swim -up method, the other one was prepared by MACS and the last one was prepared by zeta potential. Then sperm count, viability, motility and morphology were assessed according to WHO 2010. DNA damage were assessed by Sperm DNA Fragmentation assay and sperm chromatin packaging assessed by CMA3 staining testMain results and the role of chanceSperm parameters including viability, motility, and morphology in the Microfluidic method were significantly improved and sperm DNA damage were significantl...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –102 GM-CSF (granulocyte-macrophage colony-stimulating factor) as a sperm medium supplement improves sperm quality in Oligoastenoteratospermia (OAT) men by activating the PI3K/Akt pathway
This study needs further investigations, including Real-time PCR for the genes of this signaling pathway which is ongoing.Wider implications of the findings: We showed for the first time that GM-CSF can improve sperm quality by influencing motility and energy metabolism in spermatozoa which can be affected by increasing the phosphorylation of AKT. This growth factor could be an appropriate supplement in sperm media for OAT patients.Trial registration numberIRCT20200519047508N1 (Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –005 Magnetic-activated cell sorting in couples undergoing preimplantation genetic testing for aneuploidies (PGT-A) using autologous oocytes shows slightly lower aneuploidy rates compared to standard semen processing
AbstractStudy questionDoes the selection of non-apoptotic sperm via magnetic-activated cell sorting (MACS) reduce the aneuploidy rate of embryos from couples undergoing ICSI cycles with PGT-A using the patients ’ own oocytes?Summary answerIt does. The aneuploidy rate in the MACS group was 4.34% lower than the one obtained using semen samples processed according to standard clinical practice.What is known alreadyMACS is a successful tool in eliminating proapoptotic sperm from a semen sample. However, the true effect of this technique on reproductive outcomes and the quality of the resulting embryos are a matter of controv...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –004 Effect of varicocelectomy on sperm DNA fragmentation rates in infertile men with clinical varicocele: a systematic review and meta-analysis
AbstractStudy questionDoes varicocelectomy improve sperm DNA quality in men with infertility and clinically detected varicoceles?Summary answerVaricocelectomy reduces sperm DNA fragmentation (SDF) rates in infertile men with clinical varicocele.What is known alreadyVaricocele has been linked to male infertility through various non-mutually exclusive mechanisms, including an increase in reactive oxygen species (ROS) production that may lead to sperm DNA damage. Damage to sperm DNA may result in longer time-to-pregnancy, unexplained infertility, recurrent pregnancy loss, and failed intrauterine insemination or in vitro ferti...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –003 Transposon insertion profiling by sequencing (TIPseq) identifies novel LINE–1 insertions in human sperm
This study for the first time reports novel LINE–1 insertions in human sperm, demonstrating that scTIPseq method is a feasible tech nique, and identifying new contributions to genetic diversity in the human germ line. Further studies are needed to evaluate the impact of these insertions on sperm function.Trial registration numberNot applicable (Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –002 Prediction model for salvage microdissection testicular sperm extraction in patients with failed conventional testicular sperm extraction
AbstractStudy questionIs there any intra-surgical parameter able to predict the outcome of salvage microdissection testicular sperm extraction (mTESE) in patients with previous failed TESE?Summary answerAmong all the variables under consideration, only the seminiferous tubules (ST) caliber pattern found at high magnification was able to significantly predict the mTESE outcome...What is known alreadySeveral studies have demonstrated that no clinical or hormonal parameters are able to predict the outcome of a salvage mTESE performed in patients with previous sperm retrieval failure (SRF). It has been previously demonstrated ...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
Author Index
(Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-065 The naughty cells of the endometriumxx
AbstractStem/progenitor cells are the naughty cells of the endometrium! The term “naughty” has a number of connotations, one being immaturity which I will apply to the rare stem/progenitor cell populations hiding in the endometrium, where they have eluded scientists for so long. Despite their rarity, these immature cells have the capability of growing up and differentiating into the functional cells of the endometrium, producing their progenies in the process. The self-willed human endometrial epithelial progenitor cells (eEPC) and mesenchymal stem cells (eMSC) first revealed themselves through their clonogenic activit...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-238 Preterm birth after recurrent pregnancy loss: A systematic review and meta-analysis
AbstractStudy questionWhat is the impact of recurrent pregnancy loss on the risk of preterm birth?Summary answerWomen with RPL were found to be at increased odds of having preterm deliveries (<37 weeks gestation) in their subsequent live pregnancies.What is known alreadyRecurrent pregnancy loss (RPL) occurs in up to 5% of all women with miscarriages. The emotional, physical, and financial burden associated with RPL is unequivocal, and over the years, much research has gone into the management of RPL. However, relatively little is known about the perinatal outcomes following RPL. Past research in the area reports conflic...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-237 A non-invasive approach for aneuploidy analysis in clinical miscarriages
This study demonstrates that genome-wide cfDNA testing in the maternal bloodstream constitutes a reliable tool to analyse chromosome aneuploidies in clinical miscarriages.What is known alreadyIt is well established that 50-70% of clinical miscarriages are caused by numerical chromosomal anomalies (aneuploidies), mostly trisomies. To date, conventional cytogenetic and advanced molecular techniques are used for the analysis of POC to identify the genetic cause of miscarriage, providing valuable information for genetic counselling. However, both approaches are based in the direct analysis of the abortive tissue, which entails...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P-295 Does endometriosis affect oocyte quality? An analysis of 13 627 donor oocyte recipient and autologous IVF cycles
AbstractStudy questionDoes endometriosis affect live birth following donor oocyte recipient versus autologous in vitro fertilisation (IVF) cycle.Summary answerThere was no significant difference in the live birth rate (LBR) in women with endometriosis undergoing donor oocyte recipient versus autologous IVF cycle.What is known alreadyFor infertile women with endometriosis, IVF is often considered as a treatment option. Lower implantation and pregnancy rates have been observed following IVF in women with endometriosis when compared to tubal factor infertility. It has been debated that lower pregnancy rates following IVF in e...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P-420 Pre-selected for an award: Uncomplicated oocyte donation pregnancies display elevated CD163 positive type 2 macrophage load in the decidua, which is associated with fetal-maternal HLA class II mismatches
AbstractStudy questionDo quantity and composition of decidual macrophages differ between uncomplicated oocyte donation (OD) pregnancies and non-OD in vitro fertilization (IVF) pregnancies?Summary answerOD placentas show higher decidual CD163 positive fraction within the total macrophage population compared to non-OD IVF placentas.What is known alreadyThe embryo of an OD pregnancy is completely allogeneic to the mother, which may lead to a bigger challenge for the maternal immune system to tolerize the fetus compared to autologous pregnancies. Placental macrophages may be essential in maintaining a healthy pregnancy. Macrop...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P-388 Pre-selected for an award: Endometrial extracellular vesicles from recurrent implantation failure patients inhibited embryonic growth and implantation via miR-6131/PAK2 pathway
AbstractStudy questionCould endometrial extracellular vesicles from recurrent implantation failure patients (RIF-EVs) attenuate the growth and implantation potentials of embryos and what are the mechanisms? Summary answerRIF-EVs inhibited embryonic growth and decreased the trophoblast functions via miR-6131/PAK2 pathway.What is known alreadyRecurrent implantation failure (RIF) is characterized by repeated embryo transfers without pregnancy. To date, the etiology of RIF remains poorly understood. Recent evidence indicated that extracellular vesicles (EVs) secreted by endometrial cells, played a crucial role in the implant...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P-382 Pre-selected for an award: Association of extended culture to blastocyst and gestational trophoblastic disease risk following IVF/ICSI assisted reproduction cycles: An analysis of large UK National database
AbstractStudy questionIs there any association between stage of embryo at transfer based on extended in vitro culture and gestational trophoblastic disease risk during assisted reproduction?Summary answerNo significant association between stages of embryo transfer from zygote stage to blastocyst stage was found after analysing 540376 cycles of IVF and ICSI.What is known alreadyGestational trophoblastic disease (GTD), commonly referred to as molar pregnancy, results from abnormal proliferation of the trophoblast with a reported incidence of ∼1 in 700 in the UK. Despite technological advances such as ICSI, PGT and selectio...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
