O-171 Altered meiotic spindle morphology and composition in in vitro matured oocytes
AbstractStudy questionHow does the meiotic spindle tubulin PTMs of MII oocytes matured in vitro compare to that of MII oocytes matured in vivo?Summary answerMII cultured in vitro present detyrosinated tubulin in the spindle microtubules, while MII oocytes matured in vivo do not.What is known alreadyA functional spindle is required for chromosomal segregation during meiosis, but the role of tubulin post-translational modifications (PTMs) in spindle meiotic dynamics remains poorly characterized. In contrast with GVs matured in vitro within the cumulus oophorous, in vitro maturation of denuded GVs to the MII stage (GV-MII) is...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-170 Predictive value of cytoplasmic granulation patterns during IVF in MII oocytes from young donors
AbstractStudy questionDo ooplasm granulation patterns of donor MII oocytes have similar predictive values for in vitro fertilization (IVF) outcomes as they have in older infertile women?Summary answerOoplasm granulation patterns of donor MII oocytes are predictive for IVF outcomes in young oocyte donors even more pronounced than in older poor prognosis patients.What is known alreadyCytoplasmic granules had been noticed for years, with data mostly focused on central granulation. Dispersed granulations were mentioned but lacked analysis.Study design, size, durationA retrospective cohort study during 2017-2020.Participants/ma...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-169 Conventional ovarian stimulation with gonadotropins depletes the developmental proteome of mouse oocytes, reducing their size and compromising their fetal yield
This study provides evidence of an additional novel effect of ex posure to gonadotropins on mouse oocyte quality, whose mechanism is mediated not by the stimulated genital tract, but by the time-dependent accumulation of proteins in oocytes.Limitations, reasons for cautionThis is an animal model study based on one mouse strain. Ovarian stimulation protocols differ between mice and humans. There can be yet other, more subtle or long-term differences between superovulated and naturally ovulated oocytes, than those described here. Proteome and transcriptome analysis cover much, but not everything.Wider implications of the fin...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-242 Metabolic coupling and spermatogenesis
AbstractAbstract textThe microenvironment of spermatogenesis mainly consists of Sertoli cells, Leydig cells, and peritubular cells. The traditional theory considers that spermatogenesis is regulated by hypothalamus-pituitary gland- gonadal axis. In the hypothalamus-pituitary gland- gonadal axis, the microenvironmental cells are mainly regulated by the hormones, which are secreted by the hypothalamus or/and pituitary gland. Meanwhile, Sertoli cells and Leydig cells also secrete related factors to feedback the functions of the hypothalamus and pituitary gland. With the development of research, it has found that metabolic dis...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-064 Artificial intelligence in embryo selection of IVF
AbstractAbstract textSome studies have discussed the use of artificial intelligence and machine learning in the assessment and selection of embryos for in vitro fertilization. Complete artificial intelligence acquired using CNN ’s dark box algorithm could be highly useful in assessing in embryos, though it could be difficult to perform the external validation necessary to confirm its value. But due to the inherent drawbacks in complete artificial intelligence assessing in vitro developmental embryos, such as lacking resu lts of discard embryos, dislocations between computer scientist and embryologist, low explanatory val...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-063 PGT-M, for multi-system genetic diseases
AbstractAbstract textReciprocal translocations (RecT) and Robertsonian translocations (RobT) are among the most common chromosomal abnormalities that cause infertility and birth defects. In 2017, the Reproductive Medicine Center of the first affiliated Hospital of Zhengzhou University reported a method named "Mapping Allele with Resolved Carrier Status" (MaReCs), which enables chromosomal ploidy screening and resolution of the translocation carrier status of the same embryo. Meanwhile, the first international healthy baby, where the chromosomal balanced translocation that can be transmitted to offspring was precisely block...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-062 In vitro maturation of oocytes in connection with ovarian tissue cryopreservation
AbstractAbstract textDiscussing fertility preservation (FP) in young cancer patients has become a key component of routine oncological health care. Although ovarian stimulation followed by oocyte cryopreservation has been recommended in cases where two to three weeks are available before the start of chemotherapy, ovarian tissue cryopreservation (OTC) is the preferred option when this timeframe is not available and when the potential gonadotoxic impact of cancer therapy is deemed moderate or severe, or in prepubertal girls. During ovarian tissue processing in the laboratory, cumulus-oocyte complexes can be identified. In v...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-161 Cumulative live birth rate after a freeze-all approach in women with polycystic ovaries: does the PCOS phenotype have an impact?
AbstractStudy questionDo cumulative live birth rates (CLBR) differ between PCOS phenotypes when a freeze-all strategy is used to prevent OHSS after ovarian stimulation (OS)?Summary answerWhen conventional-dose OS resulted in high response, a CLBR of ∼ 70% was observed after “freeze-all” in women with PCOS, irrespective of their phenotype.What is known alreadyPrevious observational studies have shown that CLBR in women with PCOS who undergo assisted reproductive technologies (ART) may depend on their phenotype. When OS was performed with caution to avoid ovarian hyperresponse, CLBR was lower in women with a hyperandro...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-160 The effect of 6-month nutritional intervention on the anthropometric, biochemical, and reproductive profile of Lebanese women with Polycystic ovarian syndrome
This study aims to assess the efficacy of, design, and implement a public health nutrition intervention designed to enhance healthy eating and life style management among PCOS patients.Summary answerAfter 6 months, this intervention decreased initial body weight by 5%-10%, increased pregnancy rate by 70%, and significantly improved psychological, metabolic and endocrine profiles.What is known alreadyPolycystic ovary syndrome (PCOS) is a common endocrinopathy among women in their reproductive age and is characterized by imbalanced hormones, irregular menses, and fertility problems. PCOS has been linked to obesity and insuli...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-159 Prediction of weight loss and drop-out in a lifestyle intervention in women with pcos: A randomized controlled trial
AbstractStudy questionWhich patient related determinants contribute to a  ≥ 5% weight loss and drop-out?Summary answerParticipating in the lifestyle treatment and a worse body image at baseline were significantly associated with ≥5% weight loss.What is known alreadyIn general, three-component interventions including diet, exercise, and cognitive behavioral therapy have shown to be effective at the long-term to achieve weight loss. In a lifestyle program for infertile women, higher external eating behavior scores and not receiving previous support by a dietician were associated with weight loss. In a short term life...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-158 The effect of circadian rhythm disruption due to constant light on ovarian and oocyte aging through possible relationship between PER2 and mTOR signaling pathway
AbstractStudy questionDoes circadian rhythm disruption by constant light affect the ovarian morphology and function, and cause ovarian and oocyte aging through possible relationship between PER2 and mTOR?Summary answerWe demonstrated that circadian rhythm disruption by light may cause ovarian and oocyte aging.What is known alreadyCircadian rhythm regulates multiple physiological processes and PER2 is one of the core circadian rhythm components. Changes in light conditions may cause circadian rhythm disruptions. Light exposure at night may cause attenuation in PER2 mRNA and protein levels. Circadian rhythm disruptions are t...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-138 Reproductive performance of women with and without intrauterine adhesions following recurrent dilatation and curettage for miscarriage: long-term follow-up of a randomized controlled trial
AbstractStudy questionAre the long-term reproductive outcomes following recurrent dilatation and curettage (D&C) for miscarriage in women with identified and treated intrauterine adhesions (IUAs) comparable to women without IUAs.Summary answerReproductive outcomes in women with identified and treated IUAs following recurrent D&C for miscarriage are impaired compared to women without IUAs.What is known alreadyThe Prevention of Adhesions Post Abortion (PAPA) study showed that application of auto-crosslinked hyaluronic acid (ACP) gel, an absorbable barrier in women undergoing recurrent D&C for miscarriage resulted...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-137 Salpingectomy versus neosalpingostomy in women with hydrosalpinx: a prospective cohort study with long-term follow-up
AbstractStudy questionWhat is the comparative effectiveness between salpingectomy and neosalpingostomy in the treatment of bilateral severe hydrosalpinx?Summary answerIn women with bilateral severe hydrosalpinx, salpingectomy combined with In Vitro Fertilization (IVF) treatment resulted in a higher cumulative live birth rate than neosalpingostomy.What is known alreadyHydrosalpinx accounts for 25% to 35% of female subfertility and has a negative effect on pregnancy rates in women who undergo IVF. So far salpingectomy before in vitro fertilization treatment has been suggested for the treatment of hydrosalpinx in order to imp...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-052 Web-based applications and online patient portals to improve patient communication
(Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-051 Telenursing within fertility
Abstract textAberdeen Fertility Centre provides fertility investigation and treatment across the north east, highlands and islands (Orkney& Shetland) of Scotland, which includes rural and remote communities. Whilst the SARS-Cov-2 pandemic has accelerated the delivery ofTele-fertility Nursing, the National Health Service (NHS) in Grampian, Scotland launched both ‘No Delays ’ and‘Near Me’ platforms in 2019 to keep pace with the digital age and with the intention of improving the quality of patient care.No Delays (series of video packages and virtual consultations) has revolutionised clinical pathways, providing i...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research