P –043 Six years’ retrospective statistical study (2013 – 2018) investigating the impact of Sperm DNA fragmentation on sperm analysis parameters
AbstractStudy questionWhat is the relationship between Sperm DNA fragmentation (SDF) levels and sperm analysis (Spermocytogramme) parameters results?Summary answerSDF level of patients with pathological spermocytogramme presents negative correlations to total spermatozoa mobility, vitality and concentration, and positive correlation to sperm morphology defects.What is known alreadyThe relationship between SDF and Sperm analysis parameters and especially sperm morphology needs to be more studied since few studies over the last years were focused on this relationship. However, abnormalities in these two parameters are consid...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –042 Impact of semen parameters, sperm DNA fragmentation and sperm aneuploidy in male infertility
AbstractStudy questionShould sperm aneuploidies and sperm DNA fragmentation (sDNAfrag) be included as valid tests in the routine investigation of male infertility?Summary answerSperm DNA fragmentation was associated with male age, oligozoospermia (OZ), oligoteratozoospermia (OT), astenoteratozoospermia (AT) and oligoastenoteratozoospermia (OAT). Sperm aneuploidies were associated with OT and OAT.What is known alreadySemen parameters assist male infertility diagnosis and treatment, but sDNAfrag and aneuploidy analysis could add useful information, as abnormal values compromise fertility. To include these tests in the routin...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –041 Reproductive concerns and sexual health in men with newly diagnosed testicular cancer prior to orchiectomy: preliminary results from an on-going study
AbstractStudy questionWhat is the prevalence of reproductive concerns among patients with newly diagnosed testicular cancer (TC), and how do they rate their sexual health (SH).Summary answerOf 20 patients, 75% (n  = 15) were moderate to highly concerned about decreased reproductive functioning. Twenty-four percent exhibited overall low SH.What is known alreadyCurrently, only little is known about reproductive concerns among TC patients. Furthermore, these concerns have not been investigated in a TC population prior to orchiectomy. One study among post-surgery TC patients two years after diagnosis, reported that 28% had...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –040 Correlation between DNA fragmentation index and big halo pattern with sperm decondensation index and semen sample parameters
AbstractStudy questionDoes sperm DNA integrity evaluated by DNA fragmentation index (DFI) and big halo pattern correlate with sperm decondensation index (SDI) and semen sample parameters?Summary answerDFI correlates with SDI and semen sample parameters in a stronger way than the big halo patternWhat is known alreadyThe sperm chromatin dispersion test evaluates DNA integrity by measuring the susceptibility of sperm DNA containing breaks to denature when treated by an acid solution. Spermatozoa with intact DNA produce big or medium size halos of dispersed DNA loops, whereas small halos or no halos indicate fragmented DNA. Th...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –039 Sperm chromatin integrity in relation to clinical pregnancy rate in an egg donation program
This study was carried out since September 2017 to March 2020Participants/materials, setting, methodsDFI was evaluated using Sperm Chromatin Dispersion test (SCD) and considering 20% or below as normal. We performed ICSI in donated eggs, cultured until blastocyst stage and transferred 1 to 3 embryos. CPR was defined as the number of patient with fetal heart beats presents in relation with the number of patients with embryo transfer. We determined CPR in both groups with normal and abnormal DFI. Fisher exact test was used to analyze the differences.Main results and the role of chanceFrom these 65 couples, in 29 male partner...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –038 Clinical outcomes of 77 testicular sperm extraction treatment cycles in non-mosaic Klinefelter syndrome patients
This study includes all patients up to 2019 presenting azoospermia due to non-mosaic Klinefelter syndrome (n  = 76) that went for infertility consultations in a private fertility clinic. Patients were evaluated by the same Urologist. The genetic analysis of the patients was performed at an academic institution. At examination patients did not refer other complaints besides infertility, and referred to have not received any hormone replacement therapy in the past.Participants/materials, setting, methodsThe 76 azoospermic patients with non-mosaic Klinefelter syndrome (KS) were treated by testicular sperm extraction (TESE...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-220 An annotation-free embryo scoring system (iDAScore ®) based on deep learning shows high performance for pregnancy prediction after single-vitrified blastocyst transfer
In this study, SVBT was performed after minimal stimulation and natural cycle in vitro fertilisation (IVF). Therefore, we had only few cycles with elective blastocyst transfer. However, there was also no bias in selecting the embryos for SVBT.Wider implications of the findingsOur results showed that objective embryo assessment by a completely automatic and annotation-free model, iDAScore, does perform as good or even better than more traditional embryo assessment or an annotation-dependent ranking tool. iDAS could be an optimal pregnancy prediction model after SVBT, especially in young and advanced age patients.Trial regis...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-219 Detailed morpho-kinetic analysis of the first cleavage can help in evaluating the viability of direct-cleaved human zygotes
This study revealed that zygotes previously classified as “direct-cleaved” and eliminated from transfer included viable zygotes which could lead to a live birth. Therefore, it is crucial to optimize the use of time-lapse imaging of human zygotes in order to precisely evaluate the first cleavage.Trial registration numbernot applicable (Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-070 Demographic impact of increased ART use in European countries
(Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-069 A demographic revolution? Social representations confronted with statistics
The objective of clinical statistics is usually to measure the efficacy of ART from a medical viewpoint, not from the standpoint of the couples’ care pathways. The gap between the two is considerable. The pathways of couples who undertake ART are marked by pitfalls that strongly affect success rates because of the risk of treatment dropout. In some countries, economic factors are a major reason for dropout because of the high cost of ART. France is a very interesting textbook case to explore this issue, as all infertility treatments are fully reimbursed for up to six artificial inseminations and four in vitro fertilizati...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P-805 Artificial oocytes: from somatic cells to fertile pups
This study demonstrates the ability to generate artificial genotyped conceptuses, yielding live offspring. The identification of a feasible donor cell, together with optimization of cell cycle stage and standardization of post-implantation development, will help promote this technique for human reproduction in couples with age-related infertility or poor ovarian reserve.Trial registration numberN/A (Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P-798 Fertility preservation in pre-pubertal boys with cancer: A three-dimensional prepubertal testicular organoid for in vitro spermatogonial stem cell propagation and spermatogenesis
This study aims to develop a 3D organoid culture system to support the proliferation of SSCs and spermatogenesis. Primary bovine ITT cells and enriched SSCs were isolated and 3D organoids were generated byin vitro culture for up to 40 days. Organoid formation was observed after using different foundation cells seeded at different densities and cultured in medium containing gonadotropic supplements.Participants/materials, setting, methodsPost-thaw bovine ITTs (2 weeks-of-age) were dissociated using two-step enzymatic digestion. Enriched SSCs were selected by Percoll gradients and differential plating. Viability and apoptosi...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P-797 A novel method for establishing human embryonic stem cells independent of feeder cells
AbstractStudy questionIs there a efficient establishing method of human embryonic stem cells directly from the human blastocysts independent of feeder cells?Summary answerWe established a novel method of generating human embryonic stem cells directly from human blastocysts independent of feeder layer cells.What is known alreadyEstablishing embryonic stem cells lines mainly needed to coculture ICM clumps with feeder cells (like mouse or human fibroblasts), this brought in potential heterogeneous pollution. Although there had be some reports about generating human ESCs independent of feeder cells, but the efficiency was low ...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P-796 Trial of Autologous Marrow derived Stem Cell Ovarian Transplantation (TAMSCOT) in young infertile women with diminished ovarian reserve for ovarian rejuvenation – HOPE still persists
This study is currently being endeavored, and women with improved ovarian reserve are followed up for any spontaneous conception or following assisted reproduction.Wider implications of the findingsThe present study demonstrates beneficial role of stem cells in improving ovarian reserve parameters in women with DOR with no acquired cause. If supported by future randomized clinical studies, it could represent a paradigm shift for fertility treatment in these women providing an opportunity to have their own biological childTrial registration numberCTRI/2020/01/022726 (Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-172 Metabolic imaging of cumulus cells to predict embryo implantation potential
AbstractStudy questionCan non-invasive metabolic imaging detect variations in cumulus cell metabolic parameters associated with a viable pregnancy of the corresponding embryo?Summary answerNoninvasive metabolic imaging can detect differences in the cumulus cell metabolic signatures between embryos that led to a viable pregnancy and those that did not.  What is known alreadyBidirectional metabolic cooperativity between the human oocyte and its surrounding cumulus cells is essential for the oocyte to acquire full developmental competency. However, the relationship between cumulus cell metabolism and oocyte viability is not ...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research