P –160 Sibling oocytes cultured in a time-lapse versus benchtop incubator: limited exposure of embryos outside the incubator improves outcomes
AbstractStudy questionDoes the limited exposure of embryos outside the incubator, during evaluation and changeover, have an impact on the blastocyst development, blastocyst quality and euploid outcomes?Summary answerExposure of embryos outside the incubator, negatively impacts the number, quality and euploidy rate of day 5 blastocysts.What is known alreadyThe laboratory environment with its culture conditions is one of the crucial elements of the delicate equation to a successful ART outcome. It has been shown that increased fluctuations in the culture conditions have a considerable impact on the number of blastocysts obta...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –159 Slow-growing embryos should be frozen on day 5
AbstractStudy questionWhat is the live birth rate after frozen embryo transfer (FET) of slow-growing embryos frozen on day 5 (D5) or on day 6 (D6)?Summary answerThe live birth rate after single FET is significantly higher for slow-growing embryos frozen on D5 compared to those frozen on D6.What is known alreadyMost data on the outcomes of blastocyst transfer stem from studies that evaluate fresh transfer from normal growing D5 blastocyst ET. However not all embryos will begin blastulation nor reach the fully expanded stage by D5; those are the slow-growing embryos. Studies that compare D5 to D6 embryos in FET cycles show c...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –158 Assisted Hatching on D + 3 in order to facilitate trophectoderm biopsy in blastocyst for PGT-A is not advisable in all patients
AbstractStudy questionIs it useful or beneficial to perform Assisted Hatching (AH) on D  + 3 previously to biopsy for PGT-A on blastocyst stage on D + 5?Summary answerThe routine use of AH on D  + 3 to facilitate the embryo biopsy on D + 5 could negatively influence the development of the embryos to blastocyst stage.What is known alreadyThe blastocyst stage is the optimal stage for performing biopsies for PGT-A, which has been reported as a key factor determining the growing clinical application of this strategy worldwide. For trophectoderm (TE) biopsy, laser-assisted drilling is used to create a zona openi...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –157 The pregnancy potential of embryos cryopreserved and thawed twice: A case-control study
This study was retrospective, and the small sample size limits interpretation of the results. FET has been demonstrated to increase the risk for fetal macrosomia and gestational hypertension/pre-eclampsia. Whether repeated cryopreservation enhances these effects or influences neonatal health in the long term needs further investigation.Wider implications of the findings: Acceptable LBR and neonatal outcomes may be expected after transfer of twice-cryopreserved embryos. Also, the survival rate is high. To avoid embryo wastage or transfer of multiple embryos, good quality surplus embryos from FET cycles may be considered for...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –156 Automatic pronuclear detection based on deep learning technology has clinical utility
In this study, we evaluated the potential clinical practicality of the automatic pronuclear detection system.Study design, size, durationEmbryos clinically evaluated between May 2018 and December 2019 by embryologists were chosen for this study. We prepared for analysis TL videos of 995 embryos that had been evaluated as having 0, 1, 2, and 3PN.Participants/materials, setting, methodsPart1:We compared the outputs of the automatic pronuclear detection system with these embryologists(three junior embryologists (1a), three intermediate embryologists (1b),and three senior embryologists (1c)) who had judged the pronuclei number...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –155 Oocyte recovery 39 hours (from 39h to 41h) after administration of follicular maturation trigger does not affect clinical results
AbstractStudy questionWhat is the clinical outcome of oocytes recovered after 39  hours from ovulation inducing drug administration?Summary answerOocytes obtained after 39  hours from follicular maturation triggering are equally viable to those obtained at the standard time of 36 hrs.What is known alreadyIn the clinical setting of ART, ovum pick-up (OPU) is generally performed around 36  hours after the administration of ovulation inducing drugs (OID). However, there are cases where OPU cannot be performed at this time often due to long operating lists. As the time elapsed between the administration of ovulation in...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –154 The role of the X Chromosome in early human embryo metabolism
This study is a reanalysis of publicly available RNA-seq data, including 1176 single cells from 59 blastocysts (24 E5, 18 E6, 17 E7) published in one study (Petropoulos et al 2016).Participants/materials, setting, methodsCells were subjected to a digital karyotype inference algorithm and aneuploid samples were removed from the dataset. Sex differential gene expression analyses (DE) were then performed in euploid trophectoderm cells (TE; 233 XY from 16 embryos and 180 XX cells from 12 embryos). Cell numbers from ICM were too sparse to compare.Main results and the role of chanceAnalysis of XX and XY TE revealed 618 significa...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –153 Comparison outcome of vitrified human embryos stored in vapor phase liquid nitrogen (LN2) and direct LN2
This study aimed to compare clinical outcomes of human embryos transfer between vapor phase and liquid LN2.Study design, size, durationThe embryo has undergone vitrification for long term storage with vapor or direct contact in LN2. After the thawing of the embryo, we checked on the survival rates. We transferred only one or two embryos per patient and kept analyzing the implantation and pregnancy ratesParticipants/materials, setting, methodsThis retrospective study was carried out from January 2018 to December 2019 with 3272cycles 4713embryos; vitrified for long term storage in vapor phase or direct contact with LN2. We c...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –152 Morphokinetic and maternal profiles of embryos derived from centrally granulated oocytes vary with their ability to implant
AbstractStudy questionDoes oocyte central granularity (CG) impact embryo morphokinetics and does this change with embryo implantation ability and maternal profile?Summary answerOocyte CG slows fertilization and cleavage morphokinetics in overall derived embryos, but not in those capable to implant, an ability associated with lower maternal age.What is known alreadyOocyte morphology is easily accessible after pre-ICSI oocyte denudation, but the implications of morphological alterations for oocyte developmental competence are not entirely known. The presence of a centrally located granular area in the ooplasm was previously ...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –151 Correlation between the first euploid frozen-thawed blastocyst embryo transfer (FBT) and the subsequent euploid FBT outcome originating from the same cohort of oocytes
AbstractStudy questionDoes the outcome of the first euploid frozen-thawed blastocyst embryo transfer affect the subsequent euploid FBT originating from the same cohort of oocytes?Summary answerThe clinical pregnancy rate and ongoing pregnancy rate of the subsequent FBT are higher if a clinical pregnancy was attained in the first euploid FBT.What is known alreadyNumerous factors including patient, cycle and embryological characteristics affect the outcome of an IVF treatment cycle. There is no data available whether the outcome of euploid FBT has an impact on the outcome of the subsequent euploid FBT of embryos originating ...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –150 Does trophectoderm biopsy performed on different blastocyst stages affect the clinical outcome?
The objective of this study is to evaluate the effect of trophectoderm (TE) biopsy on different blastocyst stages and its clinical outcome.Summary answerOur results showed that TE biopsy significantly reduced the clinical outcome of fully hatched blastocyst. What is known already: TE biopsy is a method widely practiced to harvest cells to determine the chromosomal constitution of a blastocyst, ensuring higher implantation and healthy pregnancies. The effect on clinical outcome after transferring blastocysts biopsied at different blastocysts stages has not been extensively studied.Study design, size, durationThis retrospect...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –053 Concentration of bisphenol S in human seminal plasma negatively correlates with decreasing sperm concentration in ejaculate
AbstractStudy questionIs there a relationship between concentration of bisphenol S in seminal fluid and spermiogram parameters?Summary answerBisphenol S was detected in 81% of seminal plasma samples. Negative correlation was found between BPS concentration and total sperm count in normozoospermic men.What is known alreadyHuman spermatogenesis can be influenced by a range of chemicals present in our environment. Bisphenol S (BPS) is a very frequent compound commonly used as a softener in production of plastics, where it has replaced bisphenol A. It is an endocrine disruptor frequently associated with negative effects on rep...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

P –052 Structural and functional changes in the prostate gland of men following orchitis
AbstractStudy questionThe aim of the study was to establish echometric parameters, hemodynamic and cytohistological changes in the prostate gland in men of reproductive age after orchitis.Summary answerAfter orchitis, volume and mass of the prostate increased compared to the control group, blood flow was reduced, histologically and electron microscopy changes were observed.What is known alreadyAccording to the literature, prostate diseases in men of reproductive age are an important issue in urology and andrology. The most common among them are infectious lesions of the genitourinary system, which constitute about 45%. The...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-030 Subtle tubal lesions
(Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research

O-100 The follicular fluid derived exosomes facilitate the differentiation of oocytes from human induced pluripotent stem cells through miR218 / PI3K / Akt molecular axis
AbstractAbstract withdrawn by the authors (Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research