A Bead-Based Multiplex Sandwich Immunoassay to Assess the Abundance and Posttranslational Modification State of β-Catenin

A system-wide analysis of cell signaling involves detecting and quantifying a range of proteins and their posttranslational modification states in the same cellular sample. We propose a protocol for a miniaturized, bead-based array and describe its efficiency in characterizing the different forms and functions of β-catenin. The protocol provides detailed instructions for cell culture and bead array assays that enable insights into complex networks at the systems level.

Source: Springer protocols feed by Protein Science - March 30, 2015 Category: Biochemistry Source Type: news