Three-Dimensional Electrophoresis for Quantitative Profiling of Complex Proteomes

Quantitative 2D-gel-dependent proteomics became feasible with 2D fluorescence difference gel electrophoresis (2D-DIGE), and this technique has gained wide acceptance because it has eliminated the gel to gel variations and greatly facilitated the quantitative comparisons across gels for many different experimental conditions. However, the co-migration of several proteins in the same spot is still a major limitation which detracts from the accuracy of comparative quantification and prevents unambiguous post-translational modifications (PTMs) detection.

Source: Springer protocols feed by Protein Science - March 30, 2015 Category: Biochemistry Source Type: news