In-Gel Peptide IEF Sample Preparation for LC/MS Analysis

The technique of proteolytically digesting a sample and identifying its protein components by liquid chromatography followed by mass spectrometry (LC-MS) is a widely used analytical tool. Prior fractionation by isoelectric focusing (IEF) may be performed to increase the depth of proteome coverage. Here, we describe a method for in-gel IEF separation of a proteolytic digest that utilizes commercially available immobilized pH gradient (IPG) strips and a widely used IEF instrument.

Source: Springer protocols feed by Protein Science - March 30, 2015 Category: Biochemistry Source Type: news