Investigation of heart proteome of different consomic mouse strains. Testing the effect of polymorphisms on the proteome-wide trans-variation of proteins

Publication date: Available online 27 March 2015 Source:EuPA Open Proteomics Author(s): Stefanie Forler , Oliver Klein , Sebastian Köhler , Peter N. Robinson , Henning Witt , Marc Sultan , Murat Eravci , Vera Regitz-Zagrosek , Hans Lehrach , Joachim Klose We investigated to which extent polymorphisms of an individual affect the proteomic network. Consomic mouse strains (CS) were used to study the trans-effect of the cis-variant (polymorphic) proteins of the strain PWD/Ph on the proteins of the host strain C57BL/6J. The cardiac proteome of ten CSs was analyzed by 2-DE and MS. Cis-variant PWD proteins altered a high number of C57BL/6J proteins, but the number of trans-variant proteins differed considerably between different CSs. Cardiac hypertrophy was induced in CSs. We found that high variability of the proteome, as induced by polymorphisms in CS14, acts protective against the complex disease. Graphical abstract
Source: EuPA Open Proteomics - Category: Bioinformatics Source Type: research