Kinase-two-hybrid: towards the conditional interactome

The dynamics of the protein–protein interaction network and how it responds to biological perturbations remain difficult to assay by most traditional techniques. A novel kinase-dependent yeast two-hybrid framework by Stelzl and colleagues (Grossmann et al, 2015) provides a new prism to study how tyrosine phosphorylation regulates the changes in the interactome under varying conditions.

Source: Molecular Systems Biology - March 26, 2015 Category: Molecular Biology Authors: Ochoa, D., Beltrao, P. Tags: Network Biology, Post-translational Modifications, Proteolysis & Proteomics News [amp ] Views Source Type: research