Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts

Mammalian cells encode a diverse set of Rab GTPases and their corresponding regulators. In vitro biochemical screening has proven invaluable in assigning particular Rabs as substrates for their cognate GTPase-activating proteins. However, in vitro activity does not always reflect substrate specificity in cells. This method describes a functional test of GAP activity in cells or extracts that takes into account the presence of other factors or conditions that might change observed in vitro specificity.

Source: Springer protocols feed by Biochemistry - March 26, 2015 Category: Biochemistry Source Type: news