Appearance Normalization of Histology Slides

Stains are often used to highlight distinct structures in microscopy slides of tissue samples. Frequently two stains, such as eosin and hematoxylin, are applied for purposes such as discriminating cell nuclei and cytoplasm. Standardized staining protocols help to reduce variations in staining results, however, various factors can affect stain color and intensity in practice. For example, stains can fade over time, stain colors may differ slightly, or different imaging equipment may be used.

Source: Computerized Medical Imaging and Graphics - March 25, 2015 Category: Radiology Authors: Jared Vicory, Heather D. Couture, Nancy E. Thomas, David Borland, J.S. Marron, John Woosley, Marc Niethammer Source Type: research