High-Throughput Kinetic Screening of Hybridomas to Identify High-Affinity Antibodies Using Bio-Layer Interferometry

In this study, we have applied Bio-Layer Interferometry to screen hybridoma clones based on disassociation rates using the OctetRED 384 platform. Using the OctetRED platform, we were able to screen 2000 clones within 24 hours and select clones containing high-affinity antibodies for further expansion and subsequent characterization. Using this method, we were able to identify several clones producing high-affinity antibodies that were missed by ELISA.

Source: Journal of Biomolecular Screening - March 24, 2015 Category: Molecular Biology Authors: Lad, L., Clancy, S., Kovalenko, M., Liu, C., Hui, T., Smith, V., Pagratis, N. Tags: Original Research Source Type: research