Characterization of the interactions between SIVrcm Vpx and red-capped mangabey SAMHD1

In this study, we have characterized the interactions that are essential for SIVrcm Vpx-mediated degradation of rcmSAMHD1. Using mutagenesis and molecular modeling, we have determined the key role of the 23-WLHR-26 peptide of SIVrcm Vpx in recognizing rcmSAMHD1. The amino acids F15, L36, F52, R55 and R56 at the N-terminal region of rcmSAMHD1 are involved in interaction with Vpxrcm. The molecular model of the N-terminal region of rcmSAMHD1 (rcmSAMHD1-NtD), Vpxrcm and C-terminal region of DCAF1 (DCAF1-CtD) complex further reveals that rcmSAMHD1-NtD and Vpxrcm utilizes an interaction interface that is different from that used by human SAMHD1-CtD and Vpxsm. These findings provide further insights into the different modes of interaction between Vpx and SAMHD1 as the result of the “arms race” of virus and host cell.

Source: BJ Disease - March 20, 2015 Category: Biochemistry Authors: J Li, F Xu, S Hu, J Zhou, S Mei, X Zhao, S Cen, Q Jin, C Liang, F Guo Tags: BJ Disease Source Type: research