Processing of Protein ADP-ribosylation by Nudix Hydrolases

ADP-ribosylation is a post-translational modification of proteins found in organisms from all kingdoms of life which regulates many important biological functions including DNA repair, chromatin structure, unfolded protein response, and apoptosis. Several cellular enzymes, such as macrodomain containing proteins PARG and TARG1, reverse protein ADP-ribosylation. Here we show that human Nudix-Type Motif 16 (hNUDT16) represents a new enzyme class that can process protein ADP-ribosylation in vitro, converting it into ribose-5’-phosphate tags covalently attached to the modified proteins. Furthermore, our data show that hNUDT16 enzymatic activity can be used to trim ADP-ribosylation on proteins in order to facilitate analysis of ADP-ribosylation sites on proteins by mass spectrometry.

Source: BJ Signal - March 19, 2015 Category: Biochemistry Authors: L Palazzo, B Thomas, A Jemth, T Colby, O Leidecker, K Feijs, R Zaja, O Loseva, J Carreras Puigvert, I Matic, T Helleday, I Ahel Tags: BJ Signal Source Type: research