Sam68 is cleaved by caspases under apoptotic cell death induced by ionizing radiation

In this study, we found that Sam68 protein is cleaved in immune cells undergoing apoptosis induced by -radiation. Moreover, we found that Sam68 cleavage was induced by apoptotic stimuli containing -radiation in a caspase-dependent manner. In particular, we showed that activated casepase-3, 7, 8 and 9 can directly cleave Sam68 protein through in vitro protease cleavage assay. Finally, we found that the knockdown of Sam68 attenuated -radiation–induced cell death and growth suppression. Conclusively, the cleavage of Sam68 is a new indicator for the cell damaging effects of ionizing radiation.

Source: Journal of Radiation Research - March 13, 2015 Category: Physics Authors: Cho, S.-J., Choi, M. H., Nam, S. Y., Kim, J. Y., Kim, C. S., Pyo, S., Yang, K. H. Tags: Biology Source Type: research