A Novel Chromatographic Media: Histidine-Containing Composite Cryogels for HIgG Separation from Human Serum

Histidine-containing microspheres (HCM) with 2 μm in size were synthesized by suspension polymerization of poly(hydroxyethyl methacrylate) and N-methacryloyl-l-histidine methyl ester. Then, they were used to prepare composite cryogel columns by an embedding process for affinity depletion of immunoglobulin G (HIgG) from human serum via histidine groups on microspheres. Here, we describe HIgG adsorption performance of composite cryogel columns in both aqueous solution and human serum.

Source: Springer protocols feed by Biochemistry - March 9, 2015 Category: Biochemistry Source Type: news