Aptamer-Modified Magnetic Beads in Affinity Separation of Proteins

Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given.

Source: Springer protocols feed by Biochemistry - March 9, 2015 Category: Biochemistry Source Type: news