Biocompatibility assessment and antiproliferative activity of Detarium microcarpum Guill. and Perr. fruit pulp extracts.

This study was designed to assess de biocompatibility ofDetarium microcarpumfruit extract on normal fibroblasts and its antiproliferative potentiality on human osteosarcoma MG-63 cells. Primary dermal fibroblasts and human osteosarcoma MG-63 cells were treated with different concentrations of hexane, chloroform, ethyl acetate and methanol extracts ofD. microcarpumfruit pulp for 24h, 48h and 72h. The biocompatibility property of extracts on the normal fibroblasts and its antiproliferative activity on the human osteosarcoma cells were evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide (MTT) assay. The biocompatibility study ofD. microcarpumfruit pulp showed that the chloroform extract has exhibited the highest cytotoxic effect on normal fibroblasts followed by the ethyl acetate extract. Hexane extract wasn ’t cytotoxic at concentrations of 125 and 250 µg/mL but caused more than 80 % of cell death at a concentration of 500 µg/mL. Methanol extract didn’t show a significant cytotoxic effect. Furthermore, chloroform and ethyl acetate extracts showed the best antiproliferative activity on osteosarcom a cells. A complete cell death was observed when osteosarcoma cells were treated with ethyl acetate extract at all concentrations while chloroform extract at concentrations of 250 and 500 µg/mL caused a complete cells death. Methanol extract exhibited any antiprolifarative activity. Chloroform and ethyl acetate extract of fruits pulp ofD. microcapumare...

Source: International Journal of Phytomedicine - July 10, 2021 Category: Science Authors: Rouamba Ablass é Source Type: research