Screening of secondary metabolites and immunostimulation potential of poly herbal formoulation (Asparagus racemosus, Mucuna pruriens, Anacyclus pyrethrum and Tribulus terristeris) in Wistar Albino rats

In this study, Immunomodulatory activity of Polyherbal formulation was determined. First of all, plants methanolic extracts ofAsparagus racemosus, Mucuna pruriens, Anacyclus pyrethrum, Tribulus terrestris were prepared. The methanolic extracts of each plant were taken separately in equal amount and mixed to prepare the Polyherbal formulation (PHF). Screening test was applied on PHF for quantitative analysis. Hemolytic activity, thrombolytic activity and antioxidant activity were determined on experimental animals. To demonstrate the effect of standard Immunol drug, CCl4 and polyherbal formulation extract on hematological parameters. The methanolic extract of PHF was dissolved in distilled water and the dose was selected at 100, 200 and 300 mg/kg body weight. Blood sample collected in EDTA as anticoagulant were processed to determine the effect of PHP on selected hematological parameters including hemoglobin (Hb), red blood cell counts (RBCs), white blood cells (WBCs) count and Platelets count. Liver enzymes like transaminases (ALT and AST), uric acid, urea and creatinine were determined by automated photometric method to evaluate the drug safety. Serum samples were using for the estimation of Immunoglobulin M and Immunoglobulin G using enzyme linked immunosorbent assay (ELISA). Further, the C-reactive protein (CRP) was also estimated using standard protocol. Phytochemical screening of methanolic extract of polyherbal formulation showed the presence of alkaloids, tannins, flav...
Source: International Journal of Phytomedicine - Category: Science Authors: Source Type: research