The Preparation of Chicken Kidney Cell Cultures for Virus Propagation

Chicken kidney (CK) cell cultures have historically proved useful for the assay of a number of viruses including coronaviruses. A technique for the preparation of such cell cultures, using a combination of manual and trypsin disaggregation of kidneys dissected from 2- to 3-week-old birds is described. This technique routinely gives high cell yield together with high viability and the resultant adherent primary cultures can be used for virus growth and plaque formation.
Source: Springer protocols feed by Microbiology - Category: Microbiology Source Type: news