Tall Fescue (Festuca arundinacea Schreb.)

Tall fescue (Festuca arundinacea Schreb.) is the predominant cool-season perennial grass in the United States. It is widely used for both forage and turf purposes. This chapter describes a protocol that allows for the generation of a large number of transgenic tall fescue plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calli induced from caryopsis are used as explants for inoculation with A. tumefaciens. The Agrobacterium strain used is EHA105. Hygromycin phosphotransferase gene (hph) is used as the selectable marker, and hygromycin is used as the selection agent. Calli resistant to hygromycin are obtained after 4–6 weeks of selection. Soil-grown tall fescue plants can be regenerated 4–5 months after Agrobacterium tumefaciens-mediated transformation.

Source: Springer protocols feed by Plant Sciences - January 1, 2015 Category: Biology Source Type: news