High-Throughput In Situ Hybridization: Systematical Production of Gene Expression Data and Beyond

A plethora of modern-day techniques allows the detailed characterization of the transcriptome on a quantitative level. Analyses, based on techniques such as cDNA microarrays or RNA-seq (whole transcriptome shotgun sequencing), are usually genome wide in scope and readily detect small changes in gene expression levels across different biological samples. However, when it comes to spatial localization of gene expression within the context of complex tissues, traditional methods of in situ hybridization remain unparalleled with regard to their cellular resolution.
Source: Springer protocols feed by Neuroscience - Category: Neuroscience Source Type: news