In Situ Hybridization Methods for RNA Visualization in C. elegans

A protocol is described for the detection of endogenous mRNA in whole-mount tissue using antisense RNA probes of at least 400 bases in length. The protocol takes a few days and is inexpensive, yet sensitive enough to detect low-abundance transcripts. Although optimized for the nematode C. elegans, the protocol should work on similar tissue.
Source: Springer protocols feed by Neuroscience - Category: Neuroscience Source Type: news