In vitro binding assessment and in vivo efficacy of several adsorbents against aflatoxin B1

Aflatoxins are a class of mycotoxins that impair poultry health and performance. Some clays have the ability to adsorb aflatoxins. In our study, mineralogical composition of the tested clays was confirmed by powder X-ray diffraction and simultaneous thermal analysis and their properties like pH, electrical conductivity, exchangeable cations, cation exchange capacity and clay content were determined. For their in vitro assessment regarding aflatoxin B1 (AFB1) adsorption, adsorption tests under ‘intensified conditions’ (low adsorbent and high toxin concentration) were carried out in buffers at various pH values and in real gastric juice followed by isothermal analysis in phosphate buffer. In vivo we used a completely randomised design with 4 replicate pens of 5 chicks assigned to each dietary treatment from hatch to 21 days. Dietary treatments included a negative and a positive control diet (2 mg/kg AFB1), and treatment groups receiving 2 mg/kg AFB1 and 0.5% of one of 8 adsorbents. Results of in vitro experiments ranked the adsorbents as ‘good’ (R, MB, B7, M32, M34, M5; 6 bentonites containing a cis-vacant smectite), ‘average’ (bentonite C2 containing a trans-vacant smectite) or ‘poor’ (zeolite Z08, containing clinoptilolite). The addition of AFB1 significantly reduced feed intake and/or body weight gain of the chicks compared to the negative control and to the treatment groups, except for C2 and Z08. Except Z08, all adsorbents numerically decreased the liver we...
Source: World Mycotoxin Journal - Category: Toxicology Tags: World Mycotoxin Journal Source Type: research