Neural stem cells in the adult olfactory bulb core generate mature neurons in  vivo

Local neural stem cells (NSCs) contribute to neurogenesis (thick arrows) and gliogenesis (thin arrows) in the adult olfactory bulb. EPL, external plexiform layer; GCL, granule cell layer; GL, glomerular layer; ML, mitral layer. AbstractAlthough previous studies suggest that neural stem cells (NSCs) exist in the adult olfactory bulb (OB), their location, identity, and capacity to generate mature neurons in  vivo has been little explored. Here, we injected enhanced green fluorescent protein (EGFP)-expressing retroviral particles into the OB core of adult mice to label dividing cells and to track the differentiation/maturation of any neurons they might generate. EGFP-labeled cells initially expressed adult NSC markers on days 1 to 3 postinjection (dpi), including Nestin, GLAST, Sox2, Prominin-1, and GFAP. EGFP+-doublecortin (DCX) cells with a migratory morphology were also detected and their abundance increased over a 7-day period. Furthermore, EGFP-labeled cells progressively became NeuN+ neurons, they acquired neuronal morphologies, and they became immunoreactive for OB neuron subtype markers, the most abundant representing calretinin expressing interneurons. OB-NSCs also generated glial cells, suggesting they could be multipotent in  vivo. Significantly, the newly generated neurons established and received synaptic contacts, and they expressed presynaptic proteins and the transcription factor pCREB. By contrast, when the retroviral particles were injected into the subven...

Source: Stem Cells - May 17, 2021 Category: Stem Cells Authors: Çağla Defteralı, Mireia Moreno‐Estellés, Carlos Crespo, Eva Díaz‐Guerra, María Díaz‐Moreno, Eva Vergaño‐Vera, Vanesa Nieto‐Estévez, Anahí Hurtado‐Chong, Antonella Consiglio, Helena Mira, Carlos Vicario Tags: Tissue ‐Specific Stem Cells Source Type: research