miR ‐351‐3p promotes rat amniotic fluid‐derived mesenchymal stromal cell proliferation via targeting the coding sequence of Abca4

From left to right, we show that amniotic fluid (AF) was isolated from pregnant rats, AF-derived mesenchymal stromal cells (AFMSCs) were cultured in the laboratory, highly proliferative AFMSCs were determined, RNA sequencing was conducted, and biological experiments and transcription data were integrated to unveil the regulator of AFMSC proliferation, which was found to be a novel proliferation-related factor. AbstractAmniotic fluid-derived mesenchymal stromal cells (AFMSCs) present different features, depending on the isolation timing and culture conditions. The lack of uniform experimental standards hinders the comparison of results from different studies on AFMSCs. Moreover, understanding the molecular mechanisms that underlie the features of AFMSCs isolated at different embryonic developmental stages might allow the obtention of more viable and highly proliferative AFMSCs through genetic modification. We isolated AFMSCs from pregnant rats at embryonic day (E)12, E15, E18, and E21 and compared their cell proliferation capacity and transcriptome. The cell counting kit-8 assay and RNA sequencing revealed that E12 and E15 AFMSCs showed different characteristics from E18 and E21 AFMSCs. Therefore, AFMSCs were divided into two groups: early (E12 and E15) and late (E18 and E21) pregnancy-stage groups. Next, we screened the gene/microRNA pairAbca4/miR-351-3p that was related to cell proliferation.Abca4 knockdown/overexpression suggested that this gene represses the proliferation ...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Stem Cell Technology: Epigenetics, Genomics, Proteomics and Metabonomics Source Type: research