Assessment of 8-Methosypsoralen, Lomefloxacin, Sparfloxacin, and Pirfenidone phototoxicity in Long-Evans Rats

In this study, we validated an in vivo phototoxicity model using Long-Evans (LE) rats treated with 4 well-known phototoxic drugs, namely 8-methoxypsoralen, lomefloxacin, sparfloxacin, and pirfenidone. Daily macroscopic observations of skin and eyes, ophthalmological examinations 4 days after dosing, and blood sampling for toxicokinetics (TKs) were performed after exposure of treated animals to ultraviolet, and dose-dependent eye and/or skin reactions were noted for all compounds. Margins of safety were calculated when possible and correlated well with known relative phototoxicity of the 4 compounds. We conclude that the present in vivo phototoxicity assay using LE rats with TK analysis can be used to quantitatively predict the risk of pharmaceutical phototoxicity in humans.

Source: International Journal of Toxicology - February 17, 2015 Category: Toxicology Authors: Adachi, T., Satou, Y., Satou, H., Shibata, H., Miwa, S., Iwase, Y., Yamamoto, T., Nishida, A., Masutomi, N. Tags: Regular Articles Source Type: research