Neural stem cells in the adult olfactory bulb core generate mature neurons in vivo

Local neural stem cells (NSCs) contribute to neurogenesis (thick arrows) and gliogenesis (thin arrows) in the adult olfactory bulb. GCL, Granule cell layer; ML, Mitral layer; EPL, External plexiform layer; GL, Glomerular layer. AbstractAlthough previous studies suggest that neural stem cells (NSCs) exist in the adult olfactory bulb (OB), their location, identity and capacity to generate mature neuronsin vivo has been little explored. Here, we injected EGFP ‐expressing retroviral particles into the OB core of adult mice to label dividing cells and to track the differentiation/maturation of any neurons they might generate. EGFP labelled cells initially expressed adult NSC markers on days 1‐3 post injection (dpi), including Nestin, GLAST, Sox2, Promi nin‐1 and GFAP. EGFP+‐doublecortin (DCX) cells with a migratory morphology were also detected and their abundance increased over a 7‐day period. Furthermore, EGFP‐labeled cells progressively became NeuN+ neurons, they acquired neuronal morphologies and they became immunoreactive for OB neuron subtype markers, the most abundant representing calretinin expressing interneurons. OB ‐NSCs also generated glial cells, suggesting they could be multipotent in vivo. Significantly, the newly‐generated neurons established and received synaptic contacts, and they expressed presynaptic proteins and the transcription factor pCREB. By contrast, when the retroviral particles were injec ted into the subventricular zone (SVZ), nearly a...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Tissue ‐Specific Stem Cells Source Type: research