The exchanged EF-hands in calmodulin and troponin C chimeras impair the Ca 2+ -induced hydrophobicity and alter the interaction with orai1: a spectroscopic, thermodynamic and kinetic study

Conclusions: Using ANS dye to assess induced hydrophobicity showed that exchanging EFs for all Ca2+-bound chimeras impaired ANS fluorescence and/or binding affinity, consistent with general concepts about the inadequacy of hydrophobic exposure for chimeras. However, such ANS responses exhibited no correlation with the ability to interact with Orai-CMBD. Here, the model of 1:2 binding stoichiometry of CaM/Orai-CMBD established in solution supports the already published crystal structure.
Source: BMC Biochemistry - Category: Biochemistry Authors: Source Type: research