A novel gas chromatography mass spectrometry-based serum screening method for oral squamous cell carcinoma.

In this study, we subjected OSCC patients’ serum samples to gas chromatography/mass spectrometry (GC/MS)-based metabolomic analysis. The serum samples of 18 OSCC patients were used as a training set. Their preoperative serum metabolite levels were compared with those of healthy volunteers. In addition, the pre- and postoperative serum metabolite levels of these patients were also compared. The serum samples collected from another 17 OSCC patients and healthy volunteers were used as a validation set. In addition, 12 patients with oral diseases other than OSCC were also used to evaluate the reliability of the extracted serum metabolite biomarker candidates. The metabolites that displayed significant differences were subjected to multiple logistic regression analysis, and it was found that models based on candidate biomarker pairs possessed greater accuracy than single biomarker candidates. GC/MS-based metabolomic analysis is a promising method for detecting early-stage OSCC.
Source: Head and Neck Oncology - Category: Cancer & Oncology Source Type: research