Experimental Analysis of hFACT Action During Pol II Transcription In Vitro

We present the protocols describing how to prepare different forms of nucleosomes, including intact nucleosome, covalently conjugated nucleosome, nucleosome missing one of the two H2A/2B dimers (hexasome) and tetrasome (a nucleosome missing both H2A/2B dimers). These complexes allow analysis of various aspects of FACT’s function. These approaches and other methods described below can also be applied to the study of other chromatin remodelers and chromatin-targeted factors.

Source: Springer protocols feed by Microbiology - February 13, 2015 Category: Microbiology Source Type: news