Bone marrow ‐derived mesenchymal stem cells improve rat islet graft revascularization by upregulating ISL1

This study demonstrated that BMSCs led to an increase in ISL1 expression in islets and improved the vascular remodeling after islet transplantation in an animal model. Furthermore, the results demonstrate that ISL1 can directly regulate endogenous VEGFA production in islets. These data indicate that ISL1 could be the molecular mediator that led to the effects of BMSCs on islet revascularization. ABSTRACTRevascularization of the islet transplant is a crucial step that defines the success rate of patient recovery. Bone marrow ‐derived mesenchymal stem cells (BMSCs) have been reported to promote revascularization; however, the underlying cellular mechanism remains unclear. Moreover, our LC−MS/MS results showed that BMSCs could promote the expression of insulin gene enhancer binding protein‐1 (ISL1) in islets. ISL1 i s involved in islets proliferation and plays a potential regulatory role in the revascularization of islets. This study identifies the ISL1 protein as a potential modulator in BMSCs‐mediated revascularization of islet grafts. We demonstrated that the survival rate and insulin secretion of islets w ere increased in the presence of BMSCs, indicating that BMSCs promote islet revascularization in a coculture system and rat diabetes model. Interestingly, we also observed that the presence of BMSCs led to an increase in ISL1 and VEGFA expression in both islets and the INS‐1 rat insulinoma cell li ne.In silico protein structure modeling indicated that ISL1 is a tr...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Regenerative Medicine Source Type: research