The miR ‐200 family is required for ectodermal organ development through the regulation of the epithelial stem cell niche

In this study, we inhibited themiR ‐200 family in developing murine embryos using new technology, resulting in an expanded epithelial stem cell niche and lack of cell differentiation. Inhibition of individual miRs within themiR ‐200 cluster resulted in differential developmental and cell morphology defects.miR ‐200 inhibition increased the expression of dental epithelial stem cell markers, expanded the stem cell niche and decreased progenitor cell differentiation. RNA ‐seq. identifiedmiR ‐200 regulatory pathways involved in cell differentiation and compartmentalization of the stem cell niche. ThemiR ‐200 family regulates signaling pathways required for cell differentiation and cell cycle progression. The inhibition ofmiR ‐200 decreased the size of the lower incisor due to increased autophagy and cell death. NewmiR ‐200 targets demonstrate gene networks and pathways controlling cell differentiation and maintenance of the stem cell niche. This is the first report demonstrating how themiR ‐200 family is required for in vivo progenitor cell proliferation and differentiation.
Source: Stem Cells - Category: Stem Cells Authors: Tags: Tissue ‐Specific Stem Cells Source Type: research