Evaluation of Antibacterial Activity, Chromatographic Analysis for Rutin and Quercetin Quantification Using HPLC-UV-Vis from the Hydro-Alcoholic Extract Obtained from Bidens pilosa Linné

Novel and miniaturized technique, a dynamic sonication-assisted solvent extraction before the analysis by high performance liquid chromatography-ultraviolet visible (HPLC-UV-Vis) for isolation, identification and quantification of rutin and quercetin metabolites in alcoholic extracts from Bidens pilosa Linn é, was developed. The results showed that the flowers have a greater quantity of both analytes than other parts of the plant (e.g., leaves, stem and roots), the results were corroborated by liquid chromatography-mass spectrometry (LC-MS). In addition, the developed extraction technique against trad itional methods for metabolites extraction such as solid-liquid extraction, Soxhlet and rotating-disk sorptive extraction was compared. Under optimal conditions of extraction such as 0.3 mL min-1 of solvent flow, ethanol:water (1:1) as solvent type and 0.5 g of sample amount, it was possible to reac h 85% of recovery percentage of target analytes and a limit of detection close to 0.1 µg g-1 with a linear range of 50-400 µg g-1 were also obtained. Finally, the antibacterial evaluation of the flower extract of Bidens pilosa Linné, obtained under above optimal conditions against Gram-positive b acteria, was performed. The higher values of the inhibition diameters when using 1000 mg L-1 and significant differences among Staphylococcus aureus, Bacillus cereus, and Listeria monocytogenes were observed. The tests were performed with different microorganisms inoculated from three di...
Source: Journal of the Brazilian Chemical Society - Category: Chemistry Source Type: research