Mass spectrometry-based quantification of malignant biliary stenosis biomarkers in human bile.

Publication date: January 2015 Source:New Horizons in Translational Medicine, Volume 2, Issue 2 Author(s): Annarita Farina , Annie Adrait , Jean-Marc Dumonceau , Myriam Delhaye , Jean-Louis Frossard , Yohann Couté The differential diagnosis of biliary stenosis is a critical problem for gastroenterologists. An early identification of malignant lesions would enable the rapid resort to surgical resection which currently represents the only potentially curative option. Unfortunately, the diagnostic value of all available methods (e.g. imaging technics, standard serum biomarkers) is limited by relatively poor accuracy and negative predictive value. Recently, our group and others highlighted new potential cancer biomarkers in bile by using comparative proteomic analysis. Nevertheless, to date, only a few candidates have been verified for their diagnostic performances in discriminating between malignant and non-malignant stenoses. In addition, no data have yet been collected on the simultaneous measurement of these proteins with the intent of evaluating the diagnostic interest of a panel of biomarkers. To overcome the limitation of classical verification tools and give a new impetus to the translation of bile biomarkers into clinical diagnostics, mass spectrometry-based quantification could represent a rapid and cost-effective opportunity thanks to its capacity for multiplexed, high-throughput analysis, combined with its analytical specificity and reliable quantification....
Source: New Horizons in Translational Medicine - Category: Research Source Type: research