A synthetic mRNA cell reprogramming method using CYCLIN D1 promotes DNA repair generating improved genetically stable human induced pluripotent stem cells

In conclusion, we show synthetic mRNA transfection ofCYCLIN D1 repairs DNA during reprogramming resulting in significantly improved genetically stable footprint in human iPSC, enabling a new cell reprogramming method for more accurate and reliable generation of human iPSC for disease modeling and future clinical applications.© AlphaMed Press 2021A key challenge for clinical application of induced pluripotent stem cells (iPSC) to accurately model and treat human pathologies lies in developing a method for their generation that are genetically stable to reduce long ‐term risks of cell transplant therapy. The authors show synthetic mRNA transfection ofCYCLIN D1 repairs DNA during reprogramming resulting in significantly improved genetically stable footprint in human iPSC, enabling more accurate and reliable generation of human iPSC for disease modeling and future clinical applications.

Source: Stem Cells - February 24, 2021 Category: Stem Cells Authors: Ana Bel én Alvarez‐Palomo, Jordi Requena‐Osete, Raul Delgado‐Morales, Victoria Moreno‐Manzano, Carme Grau‐Bove, Agueda M. Tejera, Manel Juan Otero, Carme Barrot, Irene Santos‐Barriopedro, Alejandro Vaquero, Jovita Mezquita‐Pla, S Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research