Potential applications of deep learning in single ‐cell RNA sequencing analysis for cell therapy and regenerative medicine

The strategy of deep learning on scRNA ‐seq data. AbstractWhen used in cell therapy and regenerative medicine strategies, stem cells have potential to treat many previously incurable diseases. However, current application methods using stem cells are underdeveloped, as these cells are used directly regardless of their culture medium and subgroup. For example, when using mesenchymal stem cells (MSCs) in cell therapy, researchers do not consider their source and culture method nor their application angle and function (soft tissue regeneration, hard tissue regeneration, suppression of immune function, or promotion of immune function). By combining machine learning methods (such as deep learning) with data  sets obtained through single‐cell RNA sequencing (scRNA‐seq) technology, we can discover the hidden structure of these cells, predict their effects more accurately, and effectively use subpopulations with differentiation potential for stem cell therapy. scRNA‐seq technology has changed the study of transcription, because it can express single‐cell genes with single‐cell anatomical resolution. However, this powerful technology is sensitive to biological and technical noise. The subsequent data analysis can be computationally difficult for a variety of reasons, such as denoising sin gle cell data, reducing dimensionality, imputing missing values, and accounting for the zero‐inflated nature. In this review, we discussed how deep learning methods combined with scRN...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Regenerative Medicine Source Type: research