Immunohistochemistry and in situ hybridization for the diagnosis and classification of squamous lesions of the anogenital region

Distinguishing anogenital squamous intraepithelial lesions from benign conditions and mimics may be problematic. Immunohistochemistry for surrogate markers of HPV infection, such as Ki-67, p16, and ProExTM C, may aid the diagnosis in equivocal cases. The main diagnostic pitfall in the diagnosis of LSIL is the occurrence of “pseudokoilocytes” in benign squamous mucosa, which may lead to overdiagnosis. When interpreted correctly, Ki-67 is a sensitive and specific marker for dysplasia in mature squamous epithelium and is therefore useful for confirmation of LSIL and condyloma.

Source: Seminars in Diagnostic Pathology - February 4, 2015 Category: Pathology Authors: Edyta C. Pirog Source Type: research